http://2010.igem.org/wiki/index.php?title=Team:Northwestern/Project/Apoptosis&feed=atom&action=history
Team:Northwestern/Project/Apoptosis - Revision history
2024-03-28T12:30:20Z
Revision history for this page on the wiki
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http://2010.igem.org/wiki/index.php?title=Team:Northwestern/Project/Apoptosis&diff=126477&oldid=prev
Timsterxox at 18:33, 23 October 2010
2010-10-23T18:33:56Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Rz protein, a periplasmic protein, carries out the final step of the cell lysis. The mechanism still has yet to be elucidated. However, it is clear that it is crucial to the function of the cassette.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Rz protein, a periplasmic protein, carries out the final step of the cell lysis. The mechanism still has yet to be elucidated. However, it is clear that it is crucial to the function of the cassette.</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">When the Bacteriophage Lysis Cassette is activated, it will lyse the cell walls to release chitin that is produced via a slow mechanism.</ins></div></td></tr>
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Timsterxox
http://2010.igem.org/wiki/index.php?title=Team:Northwestern/Project/Apoptosis&diff=126352&oldid=prev
Timsterxox at 18:12, 23 October 2010
2010-10-23T18:12:02Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|align="left" valign="top" width="80%" style="padding: 5"|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|align="left" valign="top" width="80%" style="padding: 5"|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">We used the </del>Apoptosis <del class="diffchange diffchange-inline">Cassette by Brown </del>'<del class="diffchange diffchange-inline">08 iGEM team (K124017).</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">=='''</ins>Apoptosis'<ins class="diffchange diffchange-inline">''==</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The bacteriophage cassette includes S105 protein (Holin), R protein (Endolysin), and Rz protein, all of which act in combination to lyse the <del class="diffchange diffchange-inline">bacteria</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">We used Part BBa_K124017, Bacteriophage Lysis Cassette, created by the Brown'08 iGEM team. </ins>The bacteriophage cassette includes <ins class="diffchange diffchange-inline">the </ins>S105 protein (Holin), R protein (Endolysin), and Rz protein, all of which act in combination to lyse the <ins class="diffchange diffchange-inline">cell. We attached the cassette to a promoter for it to be activated to produce proteins for cell lysis</ins>. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Holin is a small membrane protein that produces holes in the membrane. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Holin is a small membrane protein that produces holes in the membrane<ins class="diffchange diffchange-inline">. This particular Holin protein contains the mutant S gene. Part BBa_K124014, Bacteriophage Holin Gene pS105, was also made by the Brown'08 iGEM team. The S105 gene has the initial 6 base pairs at the beginning of the wild type S gene sequence removed, which causes cell lysis to be induced at a faster rate than the wild type</ins>.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>R is an endolysin that <del class="diffchange diffchange-inline">digests and cleaves </del>the cell wall. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>R <ins class="diffchange diffchange-inline">protein </ins>is an endolysin that <ins class="diffchange diffchange-inline">degrades the peptidoglycan cell wall, resulting in lysis of the cell by cleavage of </ins>the cell wall. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Rz protein, a periplasmic protein<del class="diffchange diffchange-inline">, through reasons that are not yet totally clear</del>, carries out the final step of <del class="diffchange diffchange-inline">host </del>lysis.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Rz protein, a periplasmic protein, carries out the final step of <ins class="diffchange diffchange-inline">the cell </ins>lysis<ins class="diffchange diffchange-inline">. The mechanism still has yet to be elucidated. However, it is clear that it is crucial to the function of the cassette</ins>.</div></td></tr>
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Timsterxox
http://2010.igem.org/wiki/index.php?title=Team:Northwestern/Project/Apoptosis&diff=125774&oldid=prev
Bzhang89 at 16:28, 23 October 2010
2010-10-23T16:28:35Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!--- The Mission, Experiments ---></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!--- The Mission, Experiments ---></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>{|- style="font-size: 87%; background: #<del class="diffchange diffchange-inline">AFC7C7</del>; color: #<del class="diffchange diffchange-inline">000000</del>" cellpadding="1" cellspacing="1" bordercolor="#000000" width="75%" align="center"</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{|- style="font-size: 87%; background: #<ins class="diffchange diffchange-inline">EFCDF8</ins>; color: #<ins class="diffchange diffchange-inline">FFFFFF</ins>" cellpadding="1" cellspacing="1" bordercolor="#000000" width="75%" align="center"</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern|<font color="#<del class="diffchange diffchange-inline">FFFFFF</del>">'''Home'''</font>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern|<font color="#<ins class="diffchange diffchange-inline">2B3856</ins>">'''Home'''</font>]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Brainstorm|<font color="#<del class="diffchange diffchange-inline">FFFFFF</del>">'''Brainstorm'''</font>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Brainstorm|<font color="#<ins class="diffchange diffchange-inline">000000</ins>">'''Brainstorm'''</font>]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Team|<font color="#<del class="diffchange diffchange-inline">FFFFFF</del>">'''Team'''</font>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Team|<font color="#<ins class="diffchange diffchange-inline">000000</ins>">'''Team'''</font>]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Acknowledgements|<font color="#<del class="diffchange diffchange-inline">FFFFFF</del>">'''Acknowledgements'''</font>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Acknowledgements|<font color="#<ins class="diffchange diffchange-inline">000000</ins>">'''Acknowledgements'''</font>]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Project|<font color="#<del class="diffchange diffchange-inline">2B3856</del>">'''Project'''</font>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Project|<font color="#<ins class="diffchange diffchange-inline">000000</ins>">'''Project'''</font>]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/SideProject|<font color="#<del class="diffchange diffchange-inline">FFFFFF</del>">'''Human Practices'''</font>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/SideProject|<font color="#<ins class="diffchange diffchange-inline">000000</ins>">'''Human Practices'''</font>]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Parts|<font color="#<del class="diffchange diffchange-inline">FFFFFF</del>">'''Parts'''</font>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Parts|<font color="#<ins class="diffchange diffchange-inline">000000</ins>">'''Parts'''</font>]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Notebook|<font color="#<del class="diffchange diffchange-inline">FFFFFF</del>">'''Notebook'''</font>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Notebook|<font color="#<ins class="diffchange diffchange-inline">000000</ins>">'''Notebook'''</font>]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Calendar|<font color="#<del class="diffchange diffchange-inline">FFFFFF</del>">'''Calendar'''</font>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Calendar|<font color="#<ins class="diffchange diffchange-inline">000000</ins>">'''Calendar'''</font>]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Protocol|<font color="#<del class="diffchange diffchange-inline">FFFFFF</del>">'''Protocol'''</font>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Protocol|<font color="#<ins class="diffchange diffchange-inline">000000</ins>">'''Protocol'''</font>]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Safety|<font color="#<del class="diffchange diffchange-inline">FFFFFF</del>">'''Safety'''</font>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Safety|<font color="#<ins class="diffchange diffchange-inline">000000</ins>">'''Safety'''</font>]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Links|<font color="#<del class="diffchange diffchange-inline">FFFFFF</del>">'''Links'''</font>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Links|<font color="#<ins class="diffchange diffchange-inline">000000</ins>">'''Links'''</font>]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/References|<font color="#<del class="diffchange diffchange-inline">FFFFFF</del>">'''References'''</font>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/References|<font color="#<ins class="diffchange diffchange-inline">000000</ins>">'''References'''</font>]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Media|<font color="#<del class="diffchange diffchange-inline">FFFFFF</del>">'''Media'''</font>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Media|<font color="#<ins class="diffchange diffchange-inline">000000</ins>">'''Media'''</font>]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Contact|<font color="#<del class="diffchange diffchange-inline">FFFFFF</del>">'''Contact'''</font>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Contact|<font color="#<ins class="diffchange diffchange-inline">000000</ins>">'''Contact'''</font>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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Bzhang89
http://2010.igem.org/wiki/index.php?title=Team:Northwestern/Project/Apoptosis&diff=125499&oldid=prev
Cyu286 at 15:53, 23 October 2010
2010-10-23T15:53:42Z
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><td><a href="https://2010.igem.org/Team:Northwestern/Project/Modeling"><img width="100" class="icon" src="https://static.igem.org/mediawiki/2010/a/a7/NU_modeling.jpg"></a></td></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><td><a href="https://2010.igem.org/Team:Northwestern/Project/Chassis"><img width="100" class="icon" src="https://static.igem.org/mediawiki/2010/a/a6/Nuchassis.jpg"></a></td></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><td><a href="https://2010.igem.org/Team:Northwestern/Project/Lac"><img width="100" class="icon" src="https://static.igem.org/mediawiki/2010/1/18/Nulac.jpg"></a></td></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><td><a href="https://2010.igem.org/Team:Northwestern/Project/Apoptosis"><img width="100" class="icon" src="https://static.igem.org/mediawiki/2010/c/cb/Nuapop.jpg"></a></td></ins></div></td></tr>
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Cyu286
http://2010.igem.org/wiki/index.php?title=Team:Northwestern/Project/Apoptosis&diff=122999&oldid=prev
Cyu286 at 04:06, 23 October 2010
2010-10-23T04:06:39Z
<p></p>
<a href="http://2010.igem.org/wiki/index.php?title=Team:Northwestern/Project/Apoptosis&diff=122999&oldid=122745">Show changes</a>
Cyu286
http://2010.igem.org/wiki/index.php?title=Team:Northwestern/Project/Apoptosis&diff=122745&oldid=prev
Bzhang89 at 02:51, 23 October 2010
2010-10-23T02:51:34Z
<p></p>
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Bzhang89
http://2010.igem.org/wiki/index.php?title=Team:Northwestern/Project/Apoptosis&diff=105033&oldid=prev
Cyu286 at 21:04, 16 October 2010
2010-10-16T21:04:51Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 21:04, 16 October 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">===T4 Holin===</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">We used the Apoptosis Cassette by Brown '08 iGEM team (K124017).</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">===Endolycin===</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The bacteriophage cassette includes S105 protein (Holin), R protein (Endolysin), and Rz protein, all of which act in combination to lyse the bacteria.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">===Rz Protein===</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Holin is a small membrane protein that produces holes in the membrane. </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">===Live/Dead E</del>.<del class="diffchange diffchange-inline">Coli Assay===</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">R is an endolysin that digests and cleaves the cell wall</ins>. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Fluoresceinediacetate = stains living cells</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Rz protein, a periplasmic protein, through reasons that are not yet totally clear, carries out the final step of host lysis.</ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Ethidium Bromide = stains dead cells</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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Cyu286
http://2010.igem.org/wiki/index.php?title=Team:Northwestern/Project/Apoptosis&diff=13748&oldid=prev
Gcxmatt: /* Live/Dead E.Coli Assay */
2010-06-28T20:13:03Z
<p><span class="autocomment">Live/Dead E.Coli Assay</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 20:13, 28 June 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Contact|<span style="color:#000000;">'''Contact'''</span>]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Contact|<span style="color:#000000;">'''Contact'''</span>]]</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">===T4 Holin===</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">===Endolycin===</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">===Rz Protein===</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Live/Dead E.Coli Assay===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Live/Dead E.Coli Assay===</div></td></tr>
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Gcxmatt
http://2010.igem.org/wiki/index.php?title=Team:Northwestern/Project/Apoptosis&diff=13739&oldid=prev
Gcxmatt at 20:07, 28 June 2010
2010-06-28T20:07:08Z
<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Contact|<span style="color:#000000;">'''Contact'''</span>]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>!align="center"|[[Team:Northwestern/Contact|<span style="color:#000000;">'''Contact'''</span>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">===Live/Dead E.Coli Assay===</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Fluoresceinediacetate = stains living cells</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Ethidium Bromide = stains dead cells</ins></div></td></tr>
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Gcxmatt
http://2010.igem.org/wiki/index.php?title=Team:Northwestern/Project/Apoptosis&diff=13695&oldid=prev
Cyu286: New page: Tech Institute <html> <head> <style> body { background: white url(https://static.igem.org/mediawiki/2010/3/38/NUbackground.jpg)...
2010-06-28T19:44:41Z
<p>New page: <a href="/File:LOGOBANNER1.jpg" title="File:LOGOBANNER1.jpg">Tech Institute</a> <html> <head> <style> body { background: white url(https://static.igem.org/mediawiki/2010/3/38/NUbackground.jpg)...</p>
<p><b>New page</b></p><div>[[Image:LOGOBANNER1.jpg|936px|center|link=http://wikipedia.org|Tech Institute]]<br />
<html><br />
<head><br />
<style><br />
body {<br />
background: white url(https://static.igem.org/mediawiki/2010/3/38/NUbackground.jpg) repeat-x;<br />
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<!--- The Mission, Experiments ---><br />
{|- style="background:#FFFFFF; style="font-size: 87%;" color:#000000" cellpadding="1" cellspacing="1" bordercolor="#000000" width="97%" align="center"<br />
!align="center"|[[Team:Northwestern|<span style="color:#000000;">'''Home'''</span>]]<br />
!align="center"|[[Team:Northwestern/Brainstorm|<span style="color:#000000;">'''Brainstorm'''</span>]]<br />
!align="center"|[[Team:Northwestern/Team|<span style="color:#000000;">'''Team'''</span>]]<br />
!align="center"|[[Team:Northwestern/Acknowledgements|<span style="color:#000000;">'''Acknowledgements'''</span>]]<br />
!align="center"|[[Team:Northwestern/Project|<span style="color:#000000;">'''Project'''</span>]]<br />
!align="center"|[[Team:Northwestern/SideProject|<span style="color:#000000;">'''Side Project'''</span>]]<br />
!align="center"|[[Team:Northwestern/Parts|<span style="color:#000000;">'''Parts'''</span>]]<br />
!align="center"|[[Team:Northwestern/Notebook|<span style="color:#000000;">'''Notebook'''</span>]]<br />
!align="center"|[[Team:Northwestern/Calendar|<span style="color:#000000;">'''Calendar'''</span>]]<br />
!align="center"|[[Team:Northwestern/Protocol|<span style="color:#000000;">'''Protocol'''</span>]]<br />
!align="center"|[[Team:Northwestern/Safety|<span style="color:#000000;">'''Safety'''</span>]]<br />
!align="center"|[[Team:Northwestern/Links|<span style="color:#000000;">'''Links'''</span>]]<br />
!align="center"|[[Team:Northwestern/References|<span style="color:#000000;">'''References'''</span>]]<br />
!align="center"|[[Team:Northwestern/Media|<span style="color:#000000;">'''Media'''</span>]]<br />
!align="center"|[[Team:Northwestern/Contact|<span style="color:#000000;">'''Contact'''</span>]]<br />
|}</div>
Cyu286