Team:Northwestern/Project/Apoptosis

From 2010.igem.org

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(Live/Dead E.Coli Assay)
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===T4 Holin===
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We used the Apoptosis Cassette by Brown '08 iGEM team (K124017).
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===Endolycin===
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The bacteriophage cassette includes S105 protein (Holin), R protein (Endolysin), and Rz protein, all of which act in combination to lyse the bacteria.
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===Rz Protein===
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Holin is a small membrane protein that produces holes in the membrane.
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===Live/Dead E.Coli Assay===
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R is an endolysin that digests and cleaves the cell wall.  
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Fluoresceinediacetate = stains living cells
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Rz protein, a periplasmic protein, through reasons that are not yet totally clear, carries out the final step of host lysis.
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Ethidium Bromide = stains dead cells
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Revision as of 21:04, 16 October 2010

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We used the Apoptosis Cassette by Brown '08 iGEM team (K124017).

The bacteriophage cassette includes S105 protein (Holin), R protein (Endolysin), and Rz protein, all of which act in combination to lyse the bacteria.

Holin is a small membrane protein that produces holes in the membrane.

R is an endolysin that digests and cleaves the cell wall.

Rz protein, a periplasmic protein, through reasons that are not yet totally clear, carries out the final step of host lysis.