Team:Northwestern/Project
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Overall project
Signalling (volatile or quorum) -> chitin synthase (fast) && holin (slow)
in chitinase-KO strain e coli
Project Details
volatile cell signalling
phcB -> 3-OH PAME -> phcS -> phcR -(negative control)-> phcA
article: http://jb.asm.org/cgi/reprint/179/11/3639?view=long&pmid=9171411
non-volatile cell signalling
registry: http://partsregistry.org/Cell-cell_signalling
quorum sensing: LuxR, LuxS, LuxpR
ai-2 purification
http://www.pnas.org/content/suppl/2003/06/25/1537100100.DC1/7100SuppText.html
chitin synthase
chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311
UDP-GlcNAc -> UDP + Chitin
chitinase knockout (may not be necessary)
summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts
chitinase gene (chiA): http://www.ncbi.nlm.nih.gov/gene/947837
chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300
holin
registry: http://partsregistry.org/Cell_death
Parts
RBS BBa_B0029 TCTAGAGTTCACACAGGAAACCTACTAGATG - 0.764 BBa_B0030 TCTAGAGATTAAAGAGGAGAAATACTAGATG 0.6 - BBa_B0031 TCTAGAGTCACACAGGAAACCTACTAGATG 0.07 - BBa_B0032 TCTAGAGTCACACAGGAAAGTACTAGATG 0.3 0.376 BBa_B0033 TCTAGAGTCACACAGGACTACTAGATG 0.01 0.002 BBa_B0034 TCTAGAGAAAGAGGAGAAATACTAGATG 1 1 BBa_B0035 TCTAGAGATTAAAGAGGAGAATACTAGATG - 1.124 BBa_B0064 TCTAGAGAAAGAGGGGAAATACTAGATG 0.35 -