Team:Northwestern/Project

From 2010.igem.org

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(100px Chitin Synthase)
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=== Volatile Cell Signalling ===
 
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phcB -> 3-OH PAME -> phcS -> phcR -(negative control)-> phcA
 
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article: http://jb.asm.org/cgi/reprint/179/11/3639?view=long&pmid=9171411
 
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=== Non-Volatile Cell Signalling ===
 
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registry: http://partsregistry.org/Cell-cell_signalling
 
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quorum sensing: LuxR, LuxS, LuxpR
 
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=== AI-2 Purification ===
 
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http://www.pnas.org/content/suppl/2003/06/25/1537100100.DC1/7100SuppText.html
 
=== Chitinase Knockout (may not be necessary) ===
=== Chitinase Knockout (may not be necessary) ===
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chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300
chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300
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== Parts ==
 
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{|border="1" cellpadding="1"
 
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|+'''RBS'''
 
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|BBa_B0029 || TCTAGAGTTCACACAGGAAACCTACTAGATG ||- ||0.764
 
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|-
 
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|BBa_B0030 || TCTAGAGATTAAAGAGGAGAAATACTAGATG ||0.6 ||-
 
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|-
 
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|BBa_B0031 || TCTAGAGTCACACAGGAAACCTACTAGATG ||0.07 ||-
 
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|-
 
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|BBa_B0032 || TCTAGAGTCACACAGGAAAGTACTAGATG ||0.3 ||0.376
 
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|-
 
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|BBa_B0033 || TCTAGAGTCACACAGGACTACTAGATG ||0.01 ||0.002
 
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|BBa_B0034 || TCTAGAGAAAGAGGAGAAATACTAGATG ||1 ||1
 
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|BBa_B0035 || TCTAGAGATTAAAGAGGAGAATACTAGATG ||- ||1.124
 
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|BBa_B0064 || TCTAGAGAAAGAGGGGAAATACTAGATG ||0.35 ||-
 
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|}
 

Revision as of 04:05, 28 June 2010

Tech Institute

Home Team Project Parts Notebook Safety Calendar Protocol


Contents

Overall project

Signalling (volatile or quorum) -> chitin synthase (fast) && holin (slow)

in chitinase-KO strain e coli

Project Details

Nulac.jpg Lac Operon Control

http://openwetware.org/wiki/IPTG

Nuchitin.jpg Chitin Synthesis

chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311

Nuapop.jpg Apoptosis

registry: http://partsregistry.org/Cell_death



Chitinase Knockout (may not be necessary)

summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts

chitinase gene (chiA): http://www.ncbi.nlm.nih.gov/gene/947837

chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300