Team:Northwestern/Project

(Difference between revisions)

Revision as of 04:00, 28 June 2010

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Signalling (volatile or quorum) -> chitin synthase (fast) && holin (slow)

in chitinase-KO strain e coli

phcB -> 3-OH PAME -> phcS -> phcR -(negative control)-> phcA

quorum sensing: LuxR, LuxS, LuxpR

**RBS**
BBa_B0029	TCTAGAGTTCACACAGGAAACCTACTAGATG	-	0.764
BBa_B0030	TCTAGAGATTAAAGAGGAGAAATACTAGATG	0.6	-
BBa_B0031	TCTAGAGTCACACAGGAAACCTACTAGATG	0.07	-
BBa_B0032	TCTAGAGTCACACAGGAAAGTACTAGATG	0.3	0.376
BBa_B0033	TCTAGAGTCACACAGGACTACTAGATG	0.01	0.002
BBa_B0034	TCTAGAGAAAGAGGAGAAATACTAGATG	1	1
BBa_B0035	TCTAGAGATTAAAGAGGAGAATACTAGATG	-	1.124
BBa_B0064	TCTAGAGAAAGAGGGGAAATACTAGATG	0.35	-

@@ Line 32: / Line 32: @@
 == Project Details ==
-=== [[signalling]] Lac Repressor Signaling ===
+=== [[Image:nulac.jpg]] Lac Repressor Signaling ===
 http://openwetware.org/wiki/IPTG
+=== [Image:nuchitin.jpg]] Chitin Synthase ===
+chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311
+=== [Image:nuapop.jpg]] Holin ===
+registry: http://partsregistry.org/Cell_death
+----
 === Volatile Cell Signalling ===
@@ Line 50: / Line 60: @@
 === AI-2 Purification ===
 http://www.pnas.org/content/suppl/2003/06/25/1537100100.DC1/7100SuppText.html
-=== Chitin Synthase ===
-chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311
-UDP-GlcNAc -> UDP + Chitin
 === Chitinase Knockout (may not be necessary) ===
@@ Line 64: / Line 68: @@
 chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300
-=== Holin ===
-registry: http://partsregistry.org/Cell_death
 == Parts ==