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Team:Northwestern/Project
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== Project Details== | == Project Details== | ||
| - | === | + | === Lac Repressor Signaling === |
| + | |||
| + | http://openwetware.org/wiki/IPTG | ||
| + | |||
| + | === Volatile Cell Signalling === | ||
phcB -> 3-OH PAME -> phcS -> phcR -(negative control)-> phcA | phcB -> 3-OH PAME -> phcS -> phcR -(negative control)-> phcA | ||
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article: http://jb.asm.org/cgi/reprint/179/11/3639?view=long&pmid=9171411 | article: http://jb.asm.org/cgi/reprint/179/11/3639?view=long&pmid=9171411 | ||
| - | === | + | === Non-Volatile Cell Signalling === |
registry: http://partsregistry.org/Cell-cell_signalling | registry: http://partsregistry.org/Cell-cell_signalling | ||
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quorum sensing: LuxR, LuxS, LuxpR | quorum sensing: LuxR, LuxS, LuxpR | ||
| - | === | + | === AI-2 Purification === |
http://www.pnas.org/content/suppl/2003/06/25/1537100100.DC1/7100SuppText.html | http://www.pnas.org/content/suppl/2003/06/25/1537100100.DC1/7100SuppText.html | ||
| - | === | + | === Chitin Synthase === |
chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311 | chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311 | ||
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UDP-GlcNAc -> UDP + Chitin | UDP-GlcNAc -> UDP + Chitin | ||
| - | === | + | === Chitinase Knockout (may not be necessary) === |
summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts | summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts | ||
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chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300 | chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300 | ||
| - | === | + | === Holin === |
registry: http://partsregistry.org/Cell_death | registry: http://partsregistry.org/Cell_death | ||
Revision as of 19:53, 24 June 2010
| Home | Team | Project | Parts | Notebook | Safety | Calendar | Protocol |
|---|
Contents |
Overall project
Signalling (volatile or quorum) -> chitin synthase (fast) && holin (slow)
in chitinase-KO strain e coli
Project Details
Lac Repressor Signaling
http://openwetware.org/wiki/IPTG
Volatile Cell Signalling
phcB -> 3-OH PAME -> phcS -> phcR -(negative control)-> phcA
article: http://jb.asm.org/cgi/reprint/179/11/3639?view=long&pmid=9171411
Non-Volatile Cell Signalling
registry: http://partsregistry.org/Cell-cell_signalling
quorum sensing: LuxR, LuxS, LuxpR
AI-2 Purification
http://www.pnas.org/content/suppl/2003/06/25/1537100100.DC1/7100SuppText.html
Chitin Synthase
chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311
UDP-GlcNAc -> UDP + Chitin
Chitinase Knockout (may not be necessary)
summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts
chitinase gene (chiA): http://www.ncbi.nlm.nih.gov/gene/947837
chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300
Holin
registry: http://partsregistry.org/Cell_death
Parts
| BBa_B0029 | TCTAGAGTTCACACAGGAAACCTACTAGATG | - | 0.764 |
| BBa_B0030 | TCTAGAGATTAAAGAGGAGAAATACTAGATG | 0.6 | - |
| BBa_B0031 | TCTAGAGTCACACAGGAAACCTACTAGATG | 0.07 | - |
| BBa_B0032 | TCTAGAGTCACACAGGAAAGTACTAGATG | 0.3 | 0.376 |
| BBa_B0033 | TCTAGAGTCACACAGGACTACTAGATG | 0.01 | 0.002 |
| BBa_B0034 | TCTAGAGAAAGAGGAGAAATACTAGATG | 1 | 1 |
| BBa_B0035 | TCTAGAGATTAAAGAGGAGAATACTAGATG | - | 1.124 |
| BBa_B0064 | TCTAGAGAAAGAGGGGAAATACTAGATG | 0.35 | - |
