Team:Northwestern/Project
From 2010.igem.org
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== Parts == | == Parts == | ||
{|RBS | {|RBS | ||
- | + | !BBa_B0029 | TCTAGAGTTCACACAGGAAACCTACTAGATG |- |0.764 | |
- | + | !BBa_B0030 | TCTAGAGATTAAAGAGGAGAAATACTAGATG | |0.6 - | |
- | + | !BBa_B0031 | TCTAGAGTCACACAGGAAACCTACTAGATG |0.07 |- | |
- | + | !BBa_B0032 | TCTAGAGTCACACAGGAAAGTACTAGATG |0.3 |0.376 | |
- | + | !BBa_B0033 | TCTAGAGTCACACAGGACTACTAGATG |0.01 |0.002 | |
- | + | !BBa_B0034 | TCTAGAGAAAGAGGAGAAATACTAGATG |1 |1 | |
- | + | !BBa_B0035 | TCTAGAGATTAAAGAGGAGAATACTAGATG |- |1.124 | |
- | + | !BBa_B0064 | TCTAGAGAAAGAGGGGAAATACTAGATG |0.35 |- | |
|} | |} |
Revision as of 18:59, 24 June 2010
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Contents |
Overall project
Signalling (volatile or quorum) -> chitin synthase (fast) && holin (slow)
in chitinase-KO strain e coli
Project Details
volatile cell signalling
phcB -> 3-OH PAME -> phcS -> phcR -(negative control)-> phcA
article: http://jb.asm.org/cgi/reprint/179/11/3639?view=long&pmid=9171411
non-volatile cell signalling
registry: http://partsregistry.org/Cell-cell_signalling
quorum sensing: LuxR, LuxS, LuxpR
ai-2 purification
http://www.pnas.org/content/suppl/2003/06/25/1537100100.DC1/7100SuppText.html
chitin synthase
chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311
UDP-GlcNAc -> UDP + Chitin
chitinase knockout (may not be necessary)
summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts
chitinase gene (chiA): http://www.ncbi.nlm.nih.gov/gene/947837
chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300
holin
registry: http://partsregistry.org/Cell_death
Parts
TCTAGAGTTCACACAGGAAACCTACTAGATG |- |0.764 | TCTAGAGATTAAAGAGGAGAAATACTAGATG | |0.6 - | TCTAGAGTCACACAGGAAACCTACTAGATG |0.07 |- | TCTAGAGTCACACAGGAAAGTACTAGATG |0.3 |0.376 | TCTAGAGTCACACAGGACTACTAGATG |0.01 |0.002 | TCTAGAGAAAGAGGAGAAATACTAGATG |1 |1 | TCTAGAGATTAAAGAGGAGAATACTAGATG |- |1.124 | TCTAGAGAAAGAGGGGAAATACTAGATG |0.35 |- |
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