Team:Northwestern/Project

From 2010.igem.org

(Difference between revisions)
(Parts)
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== Parts ==
== Parts ==
{|RBS
{|RBS
-
|-BBa_B0029 | TCTAGAGTTCACACAGGAAACCTACTAGATG |- |0.764
+
!BBa_B0029 | TCTAGAGTTCACACAGGAAACCTACTAGATG |- |0.764
-
|-BBa_B0030 | TCTAGAGATTAAAGAGGAGAAATACTAGATG | |0.6 -
+
!BBa_B0030 | TCTAGAGATTAAAGAGGAGAAATACTAGATG | |0.6 -
-
|-BBa_B0031 | TCTAGAGTCACACAGGAAACCTACTAGATG |0.07 |-
+
!BBa_B0031 | TCTAGAGTCACACAGGAAACCTACTAGATG |0.07 |-
-
|-BBa_B0032 | TCTAGAGTCACACAGGAAAGTACTAGATG |0.3 |0.376
+
!BBa_B0032 | TCTAGAGTCACACAGGAAAGTACTAGATG |0.3 |0.376
-
|-BBa_B0033 | TCTAGAGTCACACAGGACTACTAGATG |0.01 |0.002
+
!BBa_B0033 | TCTAGAGTCACACAGGACTACTAGATG |0.01 |0.002
-
|-BBa_B0034 | TCTAGAGAAAGAGGAGAAATACTAGATG |1 |1
+
!BBa_B0034 | TCTAGAGAAAGAGGAGAAATACTAGATG |1 |1
-
|-BBa_B0035 | TCTAGAGATTAAAGAGGAGAATACTAGATG |- |1.124
+
!BBa_B0035 | TCTAGAGATTAAAGAGGAGAATACTAGATG |- |1.124
-
|-BBa_B0064 | TCTAGAGAAAGAGGGGAAATACTAGATG |0.35 |-
+
!BBa_B0064 | TCTAGAGAAAGAGGGGAAATACTAGATG |0.35 |-
|}
|}

Revision as of 18:59, 24 June 2010

caption
Home Team Project Parts Notebook Safety Calendar Protocol


Contents

Overall project

Signalling (volatile or quorum) -> chitin synthase (fast) && holin (slow)

in chitinase-KO strain e coli

Project Details

volatile cell signalling

phcB -> 3-OH PAME -> phcS -> phcR -(negative control)-> phcA

article: http://jb.asm.org/cgi/reprint/179/11/3639?view=long&pmid=9171411

non-volatile cell signalling

registry: http://partsregistry.org/Cell-cell_signalling

quorum sensing: LuxR, LuxS, LuxpR

ai-2 purification

http://www.pnas.org/content/suppl/2003/06/25/1537100100.DC1/7100SuppText.html

chitin synthase

chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311

UDP-GlcNAc -> UDP + Chitin

chitinase knockout (may not be necessary)

summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts

chitinase gene (chiA): http://www.ncbi.nlm.nih.gov/gene/947837

chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300

holin

registry: http://partsregistry.org/Cell_death

Parts

TCTAGAGTTCACACAGGAAACCTACTAGATG |- |0.764 TCTAGAGATTAAAGAGGAGAAATACTAGATG | |0.6 - TCTAGAGTCACACAGGAAACCTACTAGATG |0.07 |- TCTAGAGTCACACAGGAAAGTACTAGATG |0.3 |0.376 TCTAGAGTCACACAGGACTACTAGATG |0.01 |0.002 TCTAGAGAAAGAGGAGAAATACTAGATG |1 |1 TCTAGAGATTAAAGAGGAGAATACTAGATG |- |1.124 TCTAGAGAAAGAGGGGAAATACTAGATG |0.35 |-