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Revision as of 02:21, 22 June 2010 (view source) Cyu286 (Talk | contribs) (→Project Details) ← Older edit		Revision as of 18:57, 24 June 2010 (view source) Cyu286 (Talk | contribs) Newer edit →
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	registry: http://partsregistry.org/Cell_death		registry: http://partsregistry.org/Cell_death
-	== Results ==	+	== Parts ==
		+	RBS
		+	BBa_B0029 TCTAGAGTTCACACAGGAAACCTACTAGATG - 0.764
		+	BBa_B0030 TCTAGAGATTAAAGAGGAGAAATACTAGATG 0.6 -
		+	BBa_B0031 TCTAGAGTCACACAGGAAACCTACTAGATG 0.07 -
		+	BBa_B0032 TCTAGAGTCACACAGGAAAGTACTAGATG 0.3 0.376
		+	BBa_B0033 TCTAGAGTCACACAGGACTACTAGATG 0.01 0.002
		+	BBa_B0034 TCTAGAGAAAGAGGAGAAATACTAGATG 1 1
		+	BBa_B0035 TCTAGAGATTAAAGAGGAGAATACTAGATG - 1.124
		+	BBa_B0064 TCTAGAGAAAGAGGGGAAATACTAGATG 0.35 -

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Revision as of 18:57, 24 June 2010

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Contents 1 Overall project 2 Project Details 2.1 volatile cell signalling 2.2 non-volatile cell signalling 2.3 ai-2 purification 2.4 chitin synthase 2.5 chitinase knockout (may not be necessary) 2.6 holin 3 Parts

Overall project

Signalling (volatile or quorum) -> chitin synthase (fast) && holin (slow)

in chitinase-KO strain e coli

Project Details

volatile cell signalling

phcB -> 3-OH PAME -> phcS -> phcR -(negative control)-> phcA

article: http://jb.asm.org/cgi/reprint/179/11/3639?view=long&pmid=9171411

non-volatile cell signalling

registry: http://partsregistry.org/Cell-cell_signalling

quorum sensing: LuxR, LuxS, LuxpR

ai-2 purification

http://www.pnas.org/content/suppl/2003/06/25/1537100100.DC1/7100SuppText.html

chitin synthase

chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311

UDP-GlcNAc -> UDP + Chitin

chitinase knockout (may not be necessary)

summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts

chitinase gene (chiA): http://www.ncbi.nlm.nih.gov/gene/947837

chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300

holin

registry: http://partsregistry.org/Cell_death

Parts

RBS BBa_B0029 TCTAGAGTTCACACAGGAAACCTACTAGATG - 0.764 BBa_B0030 TCTAGAGATTAAAGAGGAGAAATACTAGATG 0.6 - BBa_B0031 TCTAGAGTCACACAGGAAACCTACTAGATG 0.07 - BBa_B0032 TCTAGAGTCACACAGGAAAGTACTAGATG 0.3 0.376 BBa_B0033 TCTAGAGTCACACAGGACTACTAGATG 0.01 0.002 BBa_B0034 TCTAGAGAAAGAGGAGAAATACTAGATG 1 1 BBa_B0035 TCTAGAGATTAAAGAGGAGAATACTAGATG - 1.124 BBa_B0064 TCTAGAGAAAGAGGGGAAATACTAGATG 0.35 -