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Team:Northwestern/Project
From 2010.igem.org
(Difference between revisions)
(→chitinase knockout) |
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phcB -> 3-OH PAME -> phcS -> phcR -(negative control)-> phcA | phcB -> 3-OH PAME -> phcS -> phcR -(negative control)-> phcA | ||
| - | + | article: http://jb.asm.org/cgi/reprint/179/11/3639?view=long&pmid=9171411 | |
| - | + | ||
| - | http://jb.asm.org/cgi/reprint/179/11/3639?view=long&pmid=9171411 | + | |
=== non-volatile cell signalling === | === non-volatile cell signalling === | ||
| - | http://partsregistry.org/Cell-cell_signalling | + | registry: http://partsregistry.org/Cell-cell_signalling |
| + | |||
quorum sensing: LuxR, LuxS, LuxpR | quorum sensing: LuxR, LuxS, LuxpR | ||
| Line 38: | Line 37: | ||
=== chitin synthase === | === chitin synthase === | ||
| - | http://www.ncbi.nlm.nih.gov/gene/852311 | + | chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311 |
UDP-GlcNAc -> UDP + Chitin | UDP-GlcNAc -> UDP + Chitin | ||
| Line 44: | Line 43: | ||
=== chitinase knockout === | === chitinase knockout === | ||
| - | http://openwetware.org/wiki/Escherichia_coli/Knockouts | + | summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts |
| - | http://www.ncbi.nlm.nih.gov/gene/947837 | + | chitinase gene (chiA): http://www.ncbi.nlm.nih.gov/gene/947837 |
| - | http://ecoli.naist.jp/GB6/info.jsp?id=JW3300 | + | chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300 |
=== holin === | === holin === | ||
| - | http://partsregistry.org/Cell_death | + | registry: http://partsregistry.org/Cell_death |
== Results == | == Results == | ||
Revision as of 04:25, 18 June 2010
| Home | Team | Project | Parts | Notebook | Safety | Calendar | Protocol |
|---|
Contents |
Overall project
Signalling (volatile or quorum) -> chitin synthase (fast) && holin (slow)
in chitinase-KO strain e coli
Project Details
volatile cell signalling
phcB -> 3-OH PAME -> phcS -> phcR -(negative control)-> phcA
article: http://jb.asm.org/cgi/reprint/179/11/3639?view=long&pmid=9171411
non-volatile cell signalling
registry: http://partsregistry.org/Cell-cell_signalling
quorum sensing: LuxR, LuxS, LuxpR
chitin synthase
chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311
UDP-GlcNAc -> UDP + Chitin
chitinase knockout
summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts
chitinase gene (chiA): http://www.ncbi.nlm.nih.gov/gene/947837
chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300
holin
registry: http://partsregistry.org/Cell_death
