Team:Northwestern/Project
From 2010.igem.org
(Difference between revisions)
(→chitinase knockout) |
|||
Line 45: | Line 45: | ||
http://openwetware.org/wiki/Escherichia_coli/Knockouts | http://openwetware.org/wiki/Escherichia_coli/Knockouts | ||
+ | |||
+ | http://www.ncbi.nlm.nih.gov/gene/947837 | ||
=== holin === | === holin === |
Revision as of 04:22, 18 June 2010
Home | Team | Project | Parts | Notebook | Safety | Calendar | Protocol |
---|
Contents |
Overall project
Signalling (volatile or quorum) -> chitin synthase (fast) && holin (slow)
in chitinase-KO strain e coli
Project Details
volatile cell signalling
phcB -> 3-OH PAME -> phcS -> phcR -(negative control)-> phcA
http://www.ncbi.nlm.nih.gov/pubmed/9171411?dopt=Abstract
http://jb.asm.org/cgi/reprint/179/11/3639?view=long&pmid=9171411
non-volatile cell signalling
http://partsregistry.org/Cell-cell_signalling quorum sensing: LuxR, LuxS, LuxpR
chitin synthase
http://www.ncbi.nlm.nih.gov/gene/852311
UDP-GlcNAc -> UDP + Chitin
chitinase knockout
http://openwetware.org/wiki/Escherichia_coli/Knockouts
http://www.ncbi.nlm.nih.gov/gene/947837
holin
http://partsregistry.org/Cell_death