Team:Northwestern/Notebook

From 2010.igem.org

(Difference between revisions)
(7/1/10)
(November)
 
(292 intermediate revisions not shown)
Line 1: Line 1:
-
[[Image:LOGOBANNER1.jpg|936px|center|link=http://wikipedia.org|Tech Institute]]
+
__NOTOC__
 +
 
 +
 
<html>
<html>
-
<head>
 
<style>
<style>
-
body {
+
/* Wiki Hacks - START */
-
  background: white url(http://2010.igem.org/wiki/images/3/38/NUbackground.jpg) repeat-x;
+
#globalWrapper {
-
}
+
    background-color: transparent;
-
</style>
+
    padding-bottom:0px;
-
</head>
+
    border: none;
-
</html>
+
    }
-
<!--- The Mission, Experiments --->
+
#top-section {
-
{|- style="background:#FFFFFF; style="font-size: 87%;" color:#000000" cellpadding="1" cellspacing="1" bordercolor="#000000" width="97%" align="center"
+
    height: 0px;
-
!align="center"|[[Team:Northwestern|<span style="color:#000000;">'''Home'''</span>]]
+
    margin-top: 0px;
-
!align="center"|[[Team:Northwestern/Brainstorm|<span style="color:#000000;">'''Brainstorm'''</span>]]
+
    margin-left: auto;
-
!align="center"|[[Team:Northwestern/Team|<span style="color:#000000;">'''Team'''</span>]]
+
    margin-right: auto;
-
!align="center"|[[Team:Northwestern/Acknowledgements|<span style="color:#000000;">'''Acknowledgements'''</span>]]
+
    margin-bottom: 0 !important;
-
!align="center"|[[Team:Northwestern/Project|<span style="color:#000000;">'''Project'''</span>]]
+
    margin-bottom: 0px;
-
!align="center"|[[Team:Northwestern/SideProject|<span style="color:#000000;">'''Side Project'''</span>]]
+
    padding:0;
-
!align="center"|[[Team:Northwestern/Parts|<span style="color:#000000;">'''Parts'''</span>]]
+
    border: 1;
-
!align="center"|[[Team:Northwestern/Notebook|<span style="color:#000000;">'''Notebook'''</span>]]
+
    }
-
!align="center"|[[Team:Northwestern/Calendar|<span style="color:#000000;">'''Calendar'''</span>]]
+
#p-logo {
-
!align="center"|[[Team:Northwestern/Protocol|<span style="color:#000000;">'''Protocol'''</span>]]
+
    height:0px;
-
!align="center"|[[Team:Northwestern/Safety|<span style="color:#000000;">'''Safety'''</span>]]
+
    overflow:hidden;
-
!align="center"|[[Team:Northwestern/Links|<span style="color:#000000;">'''Links'''</span>]]
+
    border:none;
-
!align="center"|[[Team:Northwestern/References|<span style="color:#000000;">'''References'''</span>]]
+
    }
-
!align="center"|[[Team:Northwestern/Media|<span style="color:#000000;">'''Media'''</span>]]
+
#search-controls {
-
!align="center"|[[Team:Northwestern/Contact|<span style="color:#000000;">'''Contact'''</span>]]
+
    overflow:hidden;
-
|}
+
    display:none;
 +
    background: none;
 +
    position: absolute;
 +
    top: 100px;
 +
    right: 40px;
 +
    }          
-
===6/14-18/10 (Boot Camp)===
+
/* Wiki Hacks - END */
-
DNA extracted from kit
 
-
Part: [http://partsregistry.org/partsdb/get_part.cgi?part=BBa_I746200]
+
#content {
 +
    background-color: transparent;
 +
    width:99%;
 +
    position: relative;
 +
    float: center;
 +
    border: 0px;
 +
}
-
----
+
#menubar {
 +
background-color:transparent;
 +
font-size:85%;
 +
line-height:1em;
 +
position:absolute;
 +
top:10px;
 +
white-space:nowrap;
 +
width:50%;
 +
z-index:5;
 +
height:25px;
 +
overflow:hidden;
 +
}
-
===6/22/10===
+
#menubar li a {
 +
color:#000000;
 +
}
-
We poured our Kan, Amp, Tet, Kan+Amp, Kan+Tet, Amp+Tet antibiotic agar plates.
+
.left-menu {
 +
left:0;
 +
padding-left:13px;
 +
}
 +
.right-menu  {
 +
right:0;
 +
}
-
<u>Antibiotic Concentrations</u>
+
.firstHeading { display:none; }
-
*Amp: 50mg/500ml
+
}
-
*Kan: 31.97mg/500ml
 
-
*Tet: 6mg/500ml
 
-
----
 
-
===6/23/10===
+
#footer-box {
-
Resuspended 12O and 6G DNA from the iGEM Kit and transformed them into our competent cells.
+
background-color: transparent;
 +
color: #000000;
 +
border: 0px;
 +
margin:0 auto;
 +
padding:13px 5px;
 +
width:auto;
 +
margin-top:-1px;
 +
}
-
6G = r0010(inducible promoter)
+
#footer-box a {
-
12O = e0840(rbs30-gfp-2xterm)
+
        background-color: transparent;
 +
color: #000000;
 +
font-size:90%;
-
----
+
}
 +
/* Wiki Hacks - END */
 +
</style>
 +
</html>
-
===6/24/10===
+
<html>
-
Ran a gel of our PCR product to make sure that our taq polymerase master mix was working.  
+
<head>
 +
<style>
 +
body {
 +
  background: #EFCDF8; <!-- EEDCC8 url(http://2010.igem.org/wiki/images/3/38/BG1.jpg) repeat-y; -->
 +
}
 +
</style>
 +
</head>
 +
</html>
-
Selected single 12O and 6G colonies and transferred them to LB and incubated for 18 hours.
+
[[Image:LOGOBANNER1.jpg|936px|center|link=http://wikipedia.org|Tech Institute]]
-
Let 1 12O and 1 6G plate incubate at RT. Let 1 12O and 1 6G plate incubate at 37°C. Intended to observe lawn growth.
+
<!--- The Mission, Experiments --->
 +
{|- style="font-size: 87%; background: #EFCDF8; color: #FFFFFF" cellpadding="1" cellspacing="1" bordercolor="#000000" width="75%" align="center"
 +
!align="center"|[[Team:Northwestern|<font color="#2B3856">'''Home'''</font>]]
 +
!align="center"|[[Team:Northwestern/Brainstorm|<font color="#000000">'''Brainstorm'''</font>]]
 +
!align="center"|[[Team:Northwestern/Team|<font color="#000000">'''Team'''</font>]]
 +
!align="center"|[[Team:Northwestern/Acknowledgements|<font color="#000000">'''Acknowledgements'''</font>]]
 +
!align="center"|[[Team:Northwestern/Project|<font color="#000000">'''Project'''</font>]]
 +
!align="center"|[[Team:Northwestern/SideProject|<font color="#000000">'''Human Practices'''</font>]]
 +
!align="center"|[[Team:Northwestern/Parts|<font color="#000000">'''Parts'''</font>]]
 +
!align="center"|[[Team:Northwestern/Notebook|<font color="#000000">'''Notebook'''</font>]]
 +
!align="center"|[[Team:Northwestern/Calendar|<font color="#000000">'''Calendar'''</font>]]
 +
!align="center"|[[Team:Northwestern/Protocol|<font color="#000000">'''Protocol'''</font>]]
 +
!align="center"|[[Team:Northwestern/Safety|<font color="#000000">'''Safety'''</font>]]
 +
!align="center"|[[Team:Northwestern/Links|<font color="#000000">'''Links'''</font>]]
 +
!align="center"|[[Team:Northwestern/References|<font color="#000000">'''References'''</font>]]
 +
!align="center"|[[Team:Northwestern/Media|<font color="#000000">'''Media'''</font>]]
 +
!align="center"|[[Team:Northwestern/Contact|<font color="#000000">'''Contact'''</font>]]
 +
|}
-
----
+
{| align="center" border="0" width="75%"
 +
|
 +
='''Notebook'''=
-
===6/25/10===
+
[[Brainstorming April-June 2010]]
-
Low DNA yield; Plan to redo DNA extraction from Kit
+
-
Found red/pink colonies in 12O plates. Thought to be contaminations. Plates were thrown out.
+
NOTE: All work done by the undergraduate students of NU iGEM.
 +
=='''June'''==
 +
[[Week1 6/13/10-6/19/10]]
-
----
+
[[Week2 6/20/10-6/26/10]]
-
===6/28/10===
+
[[Week3 6/27/10-7/3/10]]
-
Kit to Stock
+
-
*'''1-12O''' (r0010: inducible promoter)
 
-
*'''1-6G''' (e0840: rbs30-gfp-2xterm)
+
=='''July'''==
 +
[[Week4 7/4/10-7/10/10]]
-
*'''3-20M''' (K124017: Bacteriophage Lysis Cassette S105, R, and Rz)
+
[[Week5 7/11/10-7/17/10]]
-
Plates were incubated at 37°C overnight (starting at 2:30 pm).
+
[[Week6 7/18/10-7/24/10]]
-
----
+
[[Week7 7/25/10-7/31/10]]
-
===6/29/10===
 
-
Selected single 1-12O, 1-6G, 3-20M colonies and grew them in Amp-LB broth for 18 hours (starting at 4:15 pm).
 
-
----
 
-
===6/30/10===
+
=='''August'''==
 +
[[Week8 8/1/10-8/7/10]]
-
Poured 2 Sleeves of Kan and Amp plates respectively
+
[[Week9 8/8/10-8/14/10]]
-
Met with Dr. Russin (BIF)
+
[[Week10 8/15/10-8/21/10]]
-
Sent emails regarding biofilm and biofilm imaging: Aaron Packman, Wei Zhang, Kimberly Grey
+
[[Week11 8/22/10-8/28/10]]
-
Most likely going to use water immersion Leica (Confocal Microscope) + low MP agar (enrobing technique)
+
[[Week12 8/29/10-9/4/10]]
-
Red colonies reappeared on 1-12O plates; Fluorescent Microscopy revealed negligible activity; still unsure why colonies are red.
 
-
Miniprepped 1-12O, 3-20M, 1-6G: nanodrop -> mostly below 20ng, 1 above 100ng; stored in -20°C
+
=='''September'''==
 +
[[Week13 9/5/10-9/11/10]]
-
Re-miniprepped after growing in liquid LB for 3 hours; nanodrop -> around 50ng/μL, 2 around 100ng/μL; stored in -20°C
+
[[Week14 9/12/10-9/18/10]]
-
Transferred overnight cultures into new 5ml LB broths. Incubated overnight in an angled shaker (starting from 4:20pm)
+
[[Week15 9/19/10-9/25/10]]
-
Plated 10μl, 25μl, 100μl, and 200μl (added LB up to 200μL) of the KR 1-12O overnight culture. Intend to measure the thickness of E.Coli lawn.
+
[[Week16 9/26/10-10/2/10]]
-
----
 
-
===7/1/10===
+
=='''October'''==
-
----
+
[[Week17 10/3/10-10/9/10]]
-
First weekly meeting with Professor Jewett, Leonard, and Mordacq.
+
-
Removed overnight cultures from incubator at 10:00am. Miniprep (let the elution buffer sit for 10 minutes with the DNA before centrifuging)  --> 1 around 30ng/μL, mostly around 75ng/μL.
+
[[Week18 10/10/10-10/16/10]]
-
Kit to Stock
+
[[Week19 10/17/10-10/23/10]]
-
*E0840
+
-
*E0240
+
-
*R0011
+
-
===7/2/10===
+
[[Week20 10/24/10-10/30/10]]
-
----
+
 
 +
 
 +
=='''November'''==
 +
[[Jamboree at MIT 11/05/10-11/07/10 :: Team Perspectives]]
 +
 
 +
|}

Latest revision as of 05:54, 20 March 2011


Tech Institute
Home Brainstorm Team Acknowledgements Project Human Practices Parts Notebook Calendar Protocol Safety Links References Media Contact

Notebook

Brainstorming April-June 2010

NOTE: All work done by the undergraduate students of NU iGEM.

June

Week1 6/13/10-6/19/10

Week2 6/20/10-6/26/10

Week3 6/27/10-7/3/10


July

Week4 7/4/10-7/10/10

Week5 7/11/10-7/17/10

Week6 7/18/10-7/24/10

Week7 7/25/10-7/31/10


August

Week8 8/1/10-8/7/10

Week9 8/8/10-8/14/10

Week10 8/15/10-8/21/10

Week11 8/22/10-8/28/10

Week12 8/29/10-9/4/10


September

Week13 9/5/10-9/11/10

Week14 9/12/10-9/18/10

Week15 9/19/10-9/25/10

Week16 9/26/10-10/2/10


October

Week17 10/3/10-10/9/10

Week18 10/10/10-10/16/10

Week19 10/17/10-10/23/10

Week20 10/24/10-10/30/10


November

Jamboree at MIT 11/05/10-11/07/10 :: Team Perspectives