Team:Northwestern

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(Project Description: Bacterial Skin)
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!align="center"|[[Team:Northwestern|<span style="color:#000000;">'''Home'''</span>]]
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!align="center"|[[Team:Northwestern/Brainstorm|<span style="color:#000000;">'''Brainstorm'''</span>]]
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== '''Project Description: Bacterial Skin''' ==
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:Our main project is to simulate mammalian skin using chitin in E Coli. The process was realized by four major components: Lawn Formation, Chitin Synthesis, Bacterial Apoptosis, and lac-Operon Signaling.
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:A thick bacterial lawn is to be generated by plating ydgG knock-out mutants (acquired from the Keio Collection) in an enriched agar plate. The protein product of ydgG plays an integral role in AI-2 Transport. ydgG knock-outs exhibit increased motility which should ultimately allow for a thick and level lawn.
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:Once the lawn is established, a solution of IPTG will be sprayed over the lawn to induce chitin synthesis (fast response) and apoptosis (slow response). IPTG mimics allolactose which binds to the LacI repressor, causing LacI to detach from DNA and allowing RNA Polymerase to begin transcription.
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:The 3.5kbp ChiA gene is to be extracted from the Saccharomyces Cerevisiae genome. Chitin Synthase - ChiA protein product - catalyzes the polymerization of chitin by transferring UDP-N-acetyl-D-glucosamine to an N(1,4 N-Acetyl-beta-D-glucosaminyl) to produce N+1(1,4 N-Acetyl-beta-D-glucosaminyl).
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:Apoptosis of cells will be achieved by using the bacteriophage lysis cassette built by the Brown '08 iGEM Team. The cassette includes Holin, Endolysin, and Rz Protein genes. Combined, the enzymes puncture and degrade the cell membrane; thus lysing the cell.
 
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:In mammalian skin, mitosis occurs in the basal layer of the epithelial cells from which the cells travel. As the cells move further away from the basal layer, they begin to die due to lack of nutrients. They are then filled with keratin and their cytoplasm is released, thus forming a continuously regenerating protective layer on the outer-most part of epithelial layer. Similarly, our bacterial colony will have chitin-filled cells undergo lysis in the top layer of the lawn. The critical difference is that the bacterial colony will not be internally controlled, but this is something that can be remedied, and possibly a goal for future teams.
 
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SIGNIFICANCE OF CHITIN:
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Medicinal Use:
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:wound and burn treatment/healing
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:hemostatic for orthopedic treatment of broken bones
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:viscoelastic solutions for ophthamology and orthopedic surgery
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:abdominal adhesions treatment
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:as antibacterial and antifungal agents and for treatment of mucous membranes
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:in tumor therapies
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:in micro surgery, neurosurgery
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:for treatment of chronic wounds, ulcers and bleeding (chitin powder)   
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  (http://www.gmp-chitosan.com/en/products-services/chitin.html)
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Industrial Use:
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[[Image:LOGOBANNER1.jpg|936px|center|link=http://wikipedia.org|Tech Institute]]
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:food/pharmaceutical thickener, stabilizer, paper-strengthener
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:insoluble in water and many organic solvents
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<!--- The Mission, Experiments --->
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{|- style="font-size: 87%; background: #EFCDF8; color: #FFFFFF" cellpadding="1" cellspacing="1" bordercolor="#000000" width="75%" align="center"
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!align="center"|[[Team:Northwestern|<font color="#2B3856">'''Home'''</font>]]
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!align="center"|[[Team:Northwestern/Brainstorm|<font color="#000000">'''Brainstorm'''</font>]]
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!align="center"|[[Team:Northwestern/Team|<font color="#000000">'''Team'''</font>]]
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!align="center"|[[Team:Northwestern/Acknowledgements|<font color="#000000">'''Acknowledgements'''</font>]]
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!align="center"|[[Team:Northwestern/Project|<font color="#000000">'''Project'''</font>]]
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!align="center"|[[Team:Northwestern/SideProject|<font color="#000000">'''Human Practices'''</font>]]
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!align="center"|[[Team:Northwestern/Parts|<font color="#000000">'''Parts'''</font>]]
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!align="center"|[[Team:Northwestern/Notebook|<font color="#000000">'''Notebook'''</font>]]
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!align="center"|[[Team:Northwestern/Calendar|<font color="#000000">'''Calendar'''</font>]]
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!align="center"|[[Team:Northwestern/Protocol|<font color="#000000">'''Protocol'''</font>]]
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!align="center"|[[Team:Northwestern/References|<font color="#000000">'''References'''</font>]]
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!align="center"|[[Team:Northwestern/Media|<font color="#000000">'''Media'''</font>]]
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!align="center"|[[Team:Northwestern/Contact|<font color="#000000">'''Contact'''</font>]]
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[[Image:ohgodtimithebacteriaareangry.jpg|936px|center]]
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{| align="center" border="0" width="75%" style="background:#FFFFFF"
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:agricultural, cosmetics, dietary supplement, water treatment (filter), etc
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{|cellspacing="30" border="0"
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|'''SCIN - Self-regenerating Chitin INduction'''
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:derivatives such as chitosan have just as many uses
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Chitin, found in the exoskeletons of insects and crustaceans as well as the cell walls of fungi, is one of the most abundant organic polymers in nature. Like keratin in skin, it comprises the protective outer layer of these organisms. Our goal is to generate a layer of chitin from a lawn of bacteria (Escherichia coli) in response to an external molecular cue. This cue induces chitin synthesis (fast) and cell lysis (slow), allowing for a build-up of chitin followed by cell lysis and subsequent release into the top layer of the lawn. Abrasions expose cells to the external cue for self-repair. This would create a regenerative chitin biolayer with potential medical and industrial applications.
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:biodegradable
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{|
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|align="center"|To find out more, click the links below:
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:structural (hard)
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<td><a href="http://2010.igem.org/Team:Northwestern/Project"><img width="220" class="icon" src="http://2010.igem.org/wiki/images/e/ee/Projectoverview_copy.jpg"></a></td>
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:Contemporary application:
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<td><a href="http://2010.igem.org/Team:Northwestern/Project/Modeling"><img width="65" class="icon" src="http://2010.igem.org/wiki/images/a/a7/NU_modeling.jpg"></a></td>
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<td><a href="http://2010.igem.org/Team:Northwestern/Project/Chassis"><img width="65" class="icon" src="http://2010.igem.org/wiki/images/a/a6/Nuchassis.jpg"></a></td>
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<td><a href="http://2010.igem.org/Team:Northwestern/Project/Lac"><img width="65" class="icon" src="http://2010.igem.org/wiki/images/1/18/Nulac.jpg"></a></td>
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<td><a href="http://2010.igem.org/Team:Northwestern/Project/Chitin Synthesis"><img width="65" class="icon" src="http://2010.igem.org/wiki/images/4/4e/Nuchitin.jpg"></a></td>
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<td><a href="http://2010.igem.org/Team:Northwestern/Project/Apoptosis"><img width="65" class="icon" src="http://2010.igem.org/wiki/images/c/cb/Nuapop.jpg"></a></td>
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<td align="center">Modeling</td>
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<td align="center">Chassis</td>
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<td align="center">Induction</td>
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<td align="center">Chitin</td>
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<td align="center">Apoptosis</td>
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|[[Image:PosterChild.jpg|330px|center]]
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:Internalization and Compartmentalization - and thus sequestering - of particles such as oil
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| align = "center" width="15%"|
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=='''Side Project: Magnetite Production'''==
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Our side project is to synthesize superparamagnetic magnetite nanoparticles in E Coli.
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<html>
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<div><iframe frameborder="0" width="200" height="305" src="http://www2.cbox.ws/box/?boxid=2063485&amp;boxtag=jl56tn&amp;sec=main" marginheight="2" marginwidth="2" scrolling="auto" allowtransparency="yes" name="cboxmain" style="border:#ababab 1px solid;" id="cboxmain"></iframe></div>
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Nano-manipulation of magnetite particles, which can lead to a plethora of advances, especially in research technology and biomedical technology.
 
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Understanding and utilization of magnetotaxis and/or magnetoception in bacteria.
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<a href="http://www2.clustrmaps.com/counter/maps.php?url=http://2010.igem.org/Team:Northwestern" id="clustrMapsLink" style="text-align: center; line-height: 0"><img src="http://www2.clustrmaps.com/counter/index2.php?url=http://2010.igem.org/Team:Northwestern" style="text-align: center;" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onerror="this.onerror=null; this.src='http://clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://clustrmaps.com';" />
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Possible production and use of ferrofluid, a paramagnetic or superparamagnetic fluid.
 
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Ability to create uniform nanoparticles will further nanotechnology. (biotech is nanotech that works)
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|-
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|colspan="3"|
== '''Northwestern University''' ==
== '''Northwestern University''' ==
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[http://en.wikipedia.org/wiki/Northwestern_University Northwestern University]
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Northwestern University in Evanston, IL, combines innovative teaching and pioneering research in a highly collaborative environment that transcends traditional academic boundaries.
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Our lab facilities are located at: Technological Institute 2145 Sheridan Road, Evanston, IL 60208
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Find out more about [http://www.northwestern.edu Northwestern University]
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Find out more about [http://www.mccormick.northwestern.edu/ McCormick School of Engineering]
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Find out more about [http://www.weinberg.northwestern.edu/ Weinberg College of Arts and Sciences]
=='''Sponsors'''==
=='''Sponsors'''==
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[[image:NU.jpg|200px]]   [[image:NEB.jpg|200px]]
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[[image:NU.jpg|200px]]       [[image:Weinberg.jpg|200px]]        [[image:Picture1.jpg|200px]]        [[image:NEB.jpg|200px]]       [[image:Promega.jpg|200px]]      [[image:invitrogen.jpg|200px]]        [[image:VWR.jpg|200px]]        [[image:BIF.jpg|200px]]        [[image:EPOCH.jpg|200px]]
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[[image:tit.jpg|400px]]       
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Latest revision as of 16:56, 16 February 2011


Tech Institute
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Ohgodtimithebacteriaareangry.jpg

SCIN - Self-regenerating Chitin INduction

Chitin, found in the exoskeletons of insects and crustaceans as well as the cell walls of fungi, is one of the most abundant organic polymers in nature. Like keratin in skin, it comprises the protective outer layer of these organisms. Our goal is to generate a layer of chitin from a lawn of bacteria (Escherichia coli) in response to an external molecular cue. This cue induces chitin synthesis (fast) and cell lysis (slow), allowing for a build-up of chitin followed by cell lysis and subsequent release into the top layer of the lawn. Abrasions expose cells to the external cue for self-repair. This would create a regenerative chitin biolayer with potential medical and industrial applications.

To find out more, click the links below:

Modeling Chassis Induction Chitin Apoptosis

PosterChild.jpg


Locations of visitors to this page


Northwestern University

Northwestern University in Evanston, IL, combines innovative teaching and pioneering research in a highly collaborative environment that transcends traditional academic boundaries.

Our lab facilities are located at: Technological Institute 2145 Sheridan Road, Evanston, IL 60208

Find out more about Northwestern University

Find out more about McCormick School of Engineering

Find out more about Weinberg College of Arts and Sciences

Sponsors

Error creating thumbnail: Image type not supported
Weinberg.jpg Picture1.jpg NEB.jpg Promega.jpg Invitrogen.jpg VWR.jpg BIF.jpg EPOCH.jpg

Tit.jpg