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Team:Newcastle:/Calcium Carbonate
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A cluster of five genes (''lcfA, ysiA, ysiB, etfB'', and ''etfA'') called the lcfA operon that may involved in the calcium carbonate precipitation, as well as fatty acid metabolism in ''Bacillus subtilis''. Analysis through inserional mutagenesis have shown that ''lcfA'' is not involved in calcium carbonate precipitation. Modulation of the ''lcfA'' operon using IPTG have pinpointed ''etfA'' to be essential. Further analysis thriugh RT-PCR have shown that lcfA to etfA gene is transcripted as a single transcription unit. | A cluster of five genes (''lcfA, ysiA, ysiB, etfB'', and ''etfA'') called the lcfA operon that may involved in the calcium carbonate precipitation, as well as fatty acid metabolism in ''Bacillus subtilis''. Analysis through inserional mutagenesis have shown that ''lcfA'' is not involved in calcium carbonate precipitation. Modulation of the ''lcfA'' operon using IPTG have pinpointed ''etfA'' to be essential. Further analysis thriugh RT-PCR have shown that lcfA to etfA gene is transcripted as a single transcription unit. | ||
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| + | Reference: Barabesi, c., Galizzi, A., Mastromei, G., Rossi, M., Tamburini, E., and Perito, B. 2007. ''Bacillus subtilitis'' gene cluster involved in calcium carbonate biomineralization. j. Bacteriol. '''189''':228-235. | ||
Revision as of 09:48, 11 June 2010
Calcium Carbonate
Calcium carbonate precipitation is a widespread process among bacteria. The process are controlled by two distinct pathways, namely biologically induced pathway and the biologically controlled pathway. In the biologically controlled pathway, bacteria is able to control the precipitation of calcium carbonate and deposit it intracellularly. In contrast, in the biologically induced pathway, calcium carbonate are deposited extracellularly. No specialized cell structure or specific molecular mechanism is thought to be involved. However environmential conditions were known to play a part.
A cluster of five genes (lcfA, ysiA, ysiB, etfB, and etfA) called the lcfA operon that may involved in the calcium carbonate precipitation, as well as fatty acid metabolism in Bacillus subtilis. Analysis through inserional mutagenesis have shown that lcfA is not involved in calcium carbonate precipitation. Modulation of the lcfA operon using IPTG have pinpointed etfA to be essential. Further analysis thriugh RT-PCR have shown that lcfA to etfA gene is transcripted as a single transcription unit.
Reference: Barabesi, c., Galizzi, A., Mastromei, G., Rossi, M., Tamburini, E., and Perito, B. 2007. Bacillus subtilitis gene cluster involved in calcium carbonate biomineralization. j. Bacteriol. 189:228-235.
