Team:Newcastle/solution

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Contents

Overview

...

BioBricks

  1. CaCO3/Urease
  2. yneA
  3. End of crack & signalling system
  4. Swarming
  5. Non-target-environment kill switch
  6. Glue

Parts Submitted to the Registry

Alkalinity resistance

...Concrete has high pH ...explanation, the solution, link to the lab book experiments.

Initial Research

Research
Initial Research on Filamentous cells

Characteristics / Actions

  • Alkaline resistant
  • Motility
  • Sense Oxygen (for cracks)
  • Quorum Sensing
  • UV Sensitivity
  • Produce glue/Silk
  • Produce product in response to signal


Environment

  • Fermenter (Batch)
  • External Face of Concrete
  • Inside Crack
  • Spray
  • End of crack
  • Non-target

Experiments

  1. Movement into cracks
  2. Speed of swimming in a particular direction


Parameters

  1. Speed of swimming in a particular direction (wt)
  2. Quantities of glue, s.s, CaCO3, cell density
  3. Strength (tensile) of bonding agents, filamentous cells


An e-Science Approach to Synthetic Biology

We investigate the benefits of an e-Science approach, with a focus on workflows, to synthetic biology.



 ???

  1. Grown in fermenter, rich medium
    • Rods
  2. Sporulation medium
    • spores
  3. Spray canister
    • spores
  4. Sprayed on concrete surface
    • germinate -> rods
  5. In crack
    • rods
  6. End of crack
    • Produce glue/CaCO3/silk/filaments
    • Produce signal peptides
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