Team:Newcastle/solution

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Revision as of 12:16, 30 July 2010 by Swoodhouse (Talk | contribs)

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Put explanation of alkaline resistant Bacillus subtilis growth on the wiki, maybe repeat the experiment.

Contents

Initial Research

Research

Required BioBricks

  1. CaCO3/Urease
  2. Filamentous Cells
  3. End of crack & signalling system
  4. Swarming
  5. Non-target-environment kill switch
  6. Glue


Parts Submitted to the Registry

Characteristics / Actions

  • Alkaline resistant
  • Motility
  • Sense Oxygen (for cracks)
  • Quorum Sensing
  • UV Sensitivity
  • Produce glue/Silk
  • Produce product in response to signal



Environment

  • Fermenter (Batch)
  • External Face of Concrete
  • Inside Crack
  • Spray
  • End of crack
  • Non-target

Experiments

  1. Movement into cracks
  2. Speed of swimming in a particular direction


Parameters

  1. Speed of swimming in a particular direction (wt)
  2. Quantities of glue, s.s, CaCO3, cell density
  3. Strength (tensile) of bonding agents, filamentous cells

 ???

  1. Grown in fermenter, rich medium
    • Rods
  2. Sporulation medium
    • spores
  3. Spray canister
    • spores
  4. Sprayed on concrete surface
    • germinate -> rods
  5. In crack
    • rods
  6. End of crack
    • Produce glue/CaCO3/silk/filaments
    • Produce signal peptides