Team:Newcastle/solution

From 2010.igem.org

(Difference between revisions)
(Research teams)
(Research teams)
Line 12: Line 12:
=== Research teams ===
=== Research teams ===
-
BioBrick 2 (End of crack & signaling system): Z.B.; P.H.; Y.E.; H.S.
+
BioBrick 2 (End of crack & signaling system): D.Y.; P.H.; Y.E.; H.S.
-
BioBrick 3 (Non-target-environment kill switch): S.W.; A.L.; R.B.; J.S.; D.Y.
+
BioBrick 3 (Non-target-environment kill switch): S.W.; A.L.; R.B.; J.S.  
-
BioBrick 4 (Spider silk & filamentous cells): J.S.; R.B.; S.W.; Y.E.; Z.B.[[Filamentous Cells]]
+
BioBrick 4 (Spider silk & filamentous cells): J.S.; R.B.; S.W.; Y.E.[[Filamentous Cells]]
BioBrick 5 (Glue & lysis): H.S.; A.L.; P.H.; D.Y.
BioBrick 5 (Glue & lysis): H.S.; A.L.; P.H.; D.Y.

Revision as of 14:31, 29 May 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Contents

Required BioBricks

  • 1) Sequential Programming (Richard)
  • 2) End of crack & signaling system
  • 3) Non-target-environment kill switch inc. inducible lysis
  • 4) Spider silk & filamentous cells
  • 5) Glue & lysis
  • 6) CaCO3/Urease

Research teams

BioBrick 2 (End of crack & signaling system): D.Y.; P.H.; Y.E.; H.S.

BioBrick 3 (Non-target-environment kill switch): S.W.; A.L.; R.B.; J.S.

BioBrick 4 (Spider silk & filamentous cells): J.S.; R.B.; S.W.; Y.E.Filamentous Cells

BioBrick 5 (Glue & lysis): H.S.; A.L.; P.H.; D.Y.

BioBrick 6 (CaCO3/Urease): All

Characteristics / Actions

  • Alkaline resistant
  • Motility
  • Sense Oxygen (for cracks)
  • Quorum Sensing
  • UV Sensitivity
  • Produce glue/Silk
  • Produce product in response to signal

Products

  • Spider silk
  • Glue
  • Amyloid Fiber (forms Beta-Sheet)
  • Calcium Carbonate
  • Quorum Sensing Peptides
  • Surfactin

Priorities

  • Filamentous cells
  • CaCO3
  • Quorum Sensing
  • Spider silk
  • Glue
  • Directed movement

Types of Bacillus cells

  • Motile rod cells
  • Filamentous
  • Chain of rods
  • Spores
  • Lysed
  • Biofilm
  • Dividing cell

Environment

  • Fermenter (Batch)
  • External Face of Concrete
  • Inside Crack
  • Spray
  • End of crack
  • Non-target

Experiments

  1. Movement into cracks
  2. Speed of swimming in a particular direction

Models

  1. Rate of migration down micro crack
  2. Rate of protein production
  3. Mechanical model of repair strength
  4. Whole process. Signal propagation to release glue

Concrete Questions

  • How big are micro cracks?
  • How porous is concrete?
  • How fast will concrete soak up liquid?
  • Will a surfactant speed up this process?
  • How to inspect for micro cracks:
    1. fluorescence
    2. ultra sound

Parameters

  1. Speed of swimming in a particular direction (wt)
  2. Quantities of glue, s.s, CaCO3, cell density
  3. Strength (tensile) of bonding agents, filamentous cells

 ???

  1. Grown in fermenter, rich medium
    • Rods
  2. Sporulation medium
    • spores
  3. Spray canister
    • spores
  4. Sprayed on concrete surface
    • germinate -> rods
  5. In crack
    • rods
  6. End of crack
    • Produce glue/CaCO3/silk/filaments
    • Produce signal peptides
Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon