Team:Newcastle/solution
From 2010.igem.org
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# Strength (tensile) of bonding agents, filamentous cells | # Strength (tensile) of bonding agents, filamentous cells | ||
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#* Produce glue/CaCO3/silk/filaments | #* Produce glue/CaCO3/silk/filaments | ||
#* Produce signal peptides | #* Produce signal peptides | ||
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Revision as of 13:19, 3 August 2010
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Put explanation of alkaline resistant Bacillus subtilis growth on the wiki, maybe repeat the experiment.
Contents |
Initial Research
Required BioBricks
- CaCO3/Urease
- Filamentous Cells
- End of crack & signalling system
- Swarming
- Non-target-environment kill switch
- Glue
Parts Submitted to the Registry
Characteristics / Actions
- Alkaline resistant
- Motility
- Sense Oxygen (for cracks)
- Quorum Sensing
- UV Sensitivity
- Produce glue/Silk
- Produce product in response to signal
Environment
- Fermenter (Batch)
- External Face of Concrete
- Inside Crack
- Spray
- End of crack
- Non-target
Experiments
- Movement into cracks
- Speed of swimming in a particular direction
Parameters
- Speed of swimming in a particular direction (wt)
- Quantities of glue, s.s, CaCO3, cell density
- Strength (tensile) of bonding agents, filamentous cells
???
- Grown in fermenter, rich medium
- Rods
- Sporulation medium
- spores
- Spray canister
- spores
- Sprayed on concrete surface
- germinate -> rods
- In crack
- rods
- End of crack
- Produce glue/CaCO3/silk/filaments
- Produce signal peptides