Team:Newcastle/solution

From 2010.igem.org

(Difference between revisions)
(Types of Bacillus cells)
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Put explanation of alkaline resistant ''Bacillus subtilis'' growth on the wiki, maybe repeat the experiment.
Put explanation of alkaline resistant ''Bacillus subtilis'' growth on the wiki, maybe repeat the experiment.
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=== Initial Research===  
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== Initial Research==  
[[Team:Newcastle/research|Research]]
[[Team:Newcastle/research|Research]]
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=== Required BioBricks ===
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== Required BioBricks ==
#[[Team:Newcastle/Urease|CaCO<sub>3</sub>/Urease]]
#[[Team:Newcastle/Urease|CaCO<sub>3</sub>/Urease]]
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#[[Team:Newcastle/glue|Glue]]
#[[Team:Newcastle/glue|Glue]]
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=== Characteristics / Actions ===
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==Parts Submitted to the Registry==
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== Characteristics / Actions ==
* Alkaline resistant
* Alkaline resistant
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=== Environment ===
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== Environment ==
* Fermenter (Batch)
* Fermenter (Batch)
* External Face of Concrete
* External Face of Concrete
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* Non-target
* Non-target
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=== Experiments ===
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== Experiments ==
# Movement into cracks
# Movement into cracks
# Speed of swimming in a particular direction
# Speed of swimming in a particular direction
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# Strength (tensile) of bonding agents, filamentous cells
# Strength (tensile) of bonding agents, filamentous cells
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=== ??? ===
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== ??? ==
# Grown in fermenter, rich medium
# Grown in fermenter, rich medium
#* Rods
#* Rods

Revision as of 07:01, 30 July 2010

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Put explanation of alkaline resistant Bacillus subtilis growth on the wiki, maybe repeat the experiment.

Contents

Initial Research

Research

Required BioBricks

  1. CaCO3/Urease
  2. Filamentous Cells
  3. End of crack & signalling system
  4. Swarming
  5. Non-target-environment kill switch
  6. Glue


Parts Submitted to the Registry

Characteristics / Actions

  • Alkaline resistant
  • Motility
  • Sense Oxygen (for cracks)
  • Quorum Sensing
  • UV Sensitivity
  • Produce glue/Silk
  • Produce product in response to signal



Environment

  • Fermenter (Batch)
  • External Face of Concrete
  • Inside Crack
  • Spray
  • End of crack
  • Non-target

Experiments

  1. Movement into cracks
  2. Speed of swimming in a particular direction


Parameters

  1. Speed of swimming in a particular direction (wt)
  2. Quantities of glue, s.s, CaCO3, cell density
  3. Strength (tensile) of bonding agents, filamentous cells

 ???

  1. Grown in fermenter, rich medium
    • Rods
  2. Sporulation medium
    • spores
  3. Spray canister
    • spores
  4. Sprayed on concrete surface
    • germinate -> rods
  5. In crack
    • rods
  6. End of crack
    • Produce glue/CaCO3/silk/filaments
    • Produce signal peptides