Team:Newcastle/solution

Put explanation of alkaline resistant Bacillus subtilis growth on the wiki, maybe repeat the experiment.

Initial Research

Research

Required BioBricks

1. CaCO₃/Urease
2. Filamentous Cells
3. End of crack & signalling system
4. Swarming
5. Non-target-environment kill switch
6. Glue

Characteristics / Actions

• Alkaline resistant
• Motility
• Sense Oxygen (for cracks)
• Quorum Sensing
• UV Sensitivity
• Produce glue/Silk
• Produce product in response to signal

Types of Bacillus cells

• Motile rod cells
• Filamentous
• Chain of rods
• Spores
• Lysed
• Biofilm
• Dividing cell

Environment

• Fermenter (Batch)
• External Face of Concrete
• Inside Crack
• Spray
• End of crack
• Non-target

Experiments

1. Movement into cracks
2. Speed of swimming in a particular direction

Parameters

1. Speed of swimming in a particular direction (wt)
2. Quantities of glue, s.s, CaCO3, cell density
3. Strength (tensile) of bonding agents, filamentous cells

 ???

1. Grown in fermenter, rich medium
   • Rods
2. Sporulation medium
   • spores
3. Spray canister
   • spores
4. Sprayed on concrete surface
   • germinate -> rods
5. In crack
   • rods
6. End of crack
   • Produce glue/CaCO3/silk/filaments
   • Produce signal peptides