"
Team:Newcastle/solution
From 2010.igem.org
(Difference between revisions)
Swoodhouse (Talk | contribs) |
Swoodhouse (Talk | contribs) (→Required BioBricks) |
||
| Line 3: | Line 3: | ||
=== Required BioBricks === | === Required BioBricks === | ||
| - | [[Team:Newcastle/Urease|CaCO<sub>3</sub>/Urease]] | + | #[[Team:Newcastle/Urease|CaCO<sub>3</sub>/Urease]] |
| - | [[Team:Newcastle/Filamentous Cells|Filamentous Cells]] | + | #[[Team:Newcastle/Filamentous Cells|Filamentous Cells]] |
| - | [[Team:Newcastle/End of crack & signalling system|End of crack & signalling system]] | + | #[[Team:Newcastle/End of crack & signalling system|End of crack & signalling system]] |
| - | [[Team:Newcastle/Thick cell walls|Thick cell walls]] | + | #[[Team:Newcastle/Thick cell walls|Thick cell walls]] |
| - | [[Team:Newcastle/Non-target-environment kill switch|Non-target-environment kill switch]] | + | #[[Team:Newcastle/Non-target-environment kill switch|Non-target-environment kill switch]] |
| - | [[Team:Newcastle/Spider silk|Spider silk]] | + | #[[Team:Newcastle/Spider silk|Spider silk]] |
=== Characteristics / Actions === | === Characteristics / Actions === | ||
Revision as of 16:33, 25 June 2010
| |||||||||||||
| |||||||||||||
Contents |
Required BioBricks
Characteristics / Actions
- Alkaline resistant
- Motility
- Sense Oxygen (for cracks)
- Quorum Sensing
- UV Sensitivity
- Produce glue/Silk
- Produce product in response to signal
Products
- Spider silk
- Glue
- Amyloid Fiber (forms Beta-Sheet)
- Calcium Carbonate
- Quorum Sensing Peptides
- Surfactin
Priorities
- Filamentous cells
- CaCO3
- Quorum Sensing
- Spider silk
- Glue
- Directed movement
Types of Bacillus cells
- Motile rod cells
- Filamentous
- Chain of rods
- Spores
- Lysed
- Biofilm
- Dividing cell
Environment
- Fermenter (Batch)
- External Face of Concrete
- Inside Crack
- Spray
- End of crack
- Non-target
Experiments
- Movement into cracks
- Speed of swimming in a particular direction
Models
- Rate of migration down micro crack
- Rate of protein production
- Mechanical model of repair strength
- Whole process. Signal propagation to release glue
Parameters
- Speed of swimming in a particular direction (wt)
- Quantities of glue, s.s, CaCO3, cell density
- Strength (tensile) of bonding agents, filamentous cells
???
- Grown in fermenter, rich medium
- Rods
- Sporulation medium
- spores
- Spray canister
- spores
- Sprayed on concrete surface
- germinate -> rods
- In crack
- rods
- End of crack
- Produce glue/CaCO3/silk/filaments
- Produce signal peptides
