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Team:Newcastle/glue
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The products of the 3 adjacent hfsD hfsA and hfsB genes are involved in the holdfast export. | The products of the 3 adjacent hfsD hfsA and hfsB genes are involved in the holdfast export. | ||
The anchoring of the holdfast polysaccharide is carried out by the hfa gene products. | The anchoring of the holdfast polysaccharide is carried out by the hfa gene products. | ||
| + | |||
| + | The inner membrane HfsE protein initiates glycosyltransferase by transferring | ||
| + | N-acetylglucosamine (NAG) from UDP−NAG to a lipid carrier. HfsG, a second glycosyltransferase protein, | ||
| + | transfers NAG subunits to the growing polysaccharide chain. HfsH deacetylates one or more NAG residues. The | ||
| + | HfsF protein translocates the polysaccharide chain linked to the lipid carrier across the inner membrane. The | ||
| + | polymerases HfsC and HfsI proteins link the NAG repeat units together. The holdfast polysaccharide is transferred | ||
| + | across the outer membrane by HfsA, HfsB and HfsD proteins. Hfa proteins mediate the polysaccharide attachment | ||
| + | to the cell. | ||
Revision as of 00:37, 11 June 2010
ULB-Brussels developed BioBricks for production of Caulobacter crescentus glue, and won the Best New BioBrick Part, Natural award in 2009. Caulobacter crescentus is a gram-negative bacterium.
E. coli
The hfsE hfsF hfsG hfsH genes are organized in operon and their products are required for the
minimum repeat unit holdfast synthesis while the polymerization of the repeat unit of the hold-
fast substance needs the expression of hfsC and hfsI genes.
The products of the 3 adjacent hfsD hfsA and hfsB genes are involved in the holdfast export.
The anchoring of the holdfast polysaccharide is carried out by the hfa gene products.
The inner membrane HfsE protein initiates glycosyltransferase by transferring
N-acetylglucosamine (NAG) from UDP−NAG to a lipid carrier. HfsG, a second glycosyltransferase protein, transfers NAG subunits to the growing polysaccharide chain. HfsH deacetylates one or more NAG residues. The HfsF protein translocates the polysaccharide chain linked to the lipid carrier across the inner membrane. The polymerases HfsC and HfsI proteins link the NAG repeat units together. The holdfast polysaccharide is transferred across the outer membrane by HfsA, HfsB and HfsD proteins. Hfa proteins mediate the polysaccharide attachment to the cell.
