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Team:Newcastle/Swarming
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=Swarming= | =Swarming= | ||
It is known that ''Bacillus subtilis'' (strain 168) which is generally used in the lab is unable to swarm on a solid surface eg. agar plate. This is because of two reasons which are: | It is known that ''Bacillus subtilis'' (strain 168) which is generally used in the lab is unable to swarm on a solid surface eg. agar plate. This is because of two reasons which are: | ||
| - | # Strain 168 contains a frameshift mutation in the gene named ''sfp'' which aids in post translational modification of the bacterial cyclic lipopeptide named surfactin produced by the ''srf''ABCD operon. Thus the bacteria is unable to produce surfactin | + | # Surfactin is a natural surfactant which helps in reducing surface tension and aids the bacteria to swarm on a solid or semi solid medium. Strain 168 contains a frameshift mutation in the gene named ''sfp'' which aids in post translational modification of the bacterial cyclic lipopeptide named surfactin produced by the ''srf''ABCD operon. Thus the bacteria is unable to produce surfactin. |
# Strain 168 is also unable to bio synthesize flagellum and thus has a very low amount of swarming. | # Strain 168 is also unable to bio synthesize flagellum and thus has a very low amount of swarming. | ||
Revision as of 13:19, 12 July 2010
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Swarming
It is known that Bacillus subtilis (strain 168) which is generally used in the lab is unable to swarm on a solid surface eg. agar plate. This is because of two reasons which are:
- Surfactin is a natural surfactant which helps in reducing surface tension and aids the bacteria to swarm on a solid or semi solid medium. Strain 168 contains a frameshift mutation in the gene named sfp which aids in post translational modification of the bacterial cyclic lipopeptide named surfactin produced by the srfABCD operon. Thus the bacteria is unable to produce surfactin.
- Strain 168 is also unable to bio synthesize flagellum and thus has a very low amount of swarming.
