http://2010.igem.org/wiki/index.php?title=Team:Newcastle/Spider_silk&feed=atom&action=history
Team:Newcastle/Spider silk - Revision history
2024-03-29T07:38:06Z
Revision history for this page on the wiki
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http://2010.igem.org/wiki/index.php?title=Team:Newcastle/Spider_silk&diff=15687&oldid=prev
RachelBoyd: /* Background Info */
2010-07-05T12:52:56Z
<p><span class="autocomment">Background Info</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|[[Image:Spider Web.jpg|300px]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[[Image:Golden orb web.jpg|300px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|[[Image:Spider Web.jpg|300px]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[[Image:Golden orb web.jpg|300px]]</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|[[http://www.virginiacheeseman.co.uk/giant-orb-web-nephila-madagascarensis-spiderling-p-551.html?osCsid=30c2683bb246801f57f686d92432fa20| Buy a ''Nephila'']]</ins></div></td></tr>
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RachelBoyd
http://2010.igem.org/wiki/index.php?title=Team:Newcastle/Spider_silk&diff=9586&oldid=prev
Swoodhouse: TeamNewcastle:Spider silk moved to Team:Newcastle/Spider silk
2010-06-17T09:37:59Z
<p><a href="/TeamNewcastle:Spider_silk" class="mw-redirect" title="TeamNewcastle:Spider silk">TeamNewcastle:Spider silk</a> moved to <a href="/Team:Newcastle/Spider_silk" title="Team:Newcastle/Spider silk">Team:Newcastle/Spider silk</a></p>
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<td colspan='1' style="background-color: white; color:black;">Revision as of 09:37, 17 June 2010</td>
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Swoodhouse
http://2010.igem.org/wiki/index.php?title=Team:Newcastle/Spider_silk&diff=8426&oldid=prev
Jannetta: /* Background Info */
2010-06-12T17:22:58Z
<p><span class="autocomment">Background Info</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|http://www.naturia.per.sg/buloh/inverts/nephila.htm</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|http://www.naturia.per.sg/buloh/inverts/nephila.htm</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|[[Image:Spider Web.jpg]][[Image:Golden orb web.jpg]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|[[Image:Spider Web.jpg<ins class="diffchange diffchange-inline">|300px</ins>]]<ins class="diffchange diffchange-inline">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</ins>[[Image:Golden orb web.jpg<ins class="diffchange diffchange-inline">|300px</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Major Ampulate Spidroin Proteins==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Major Ampulate Spidroin Proteins==</div></td></tr>
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Jannetta
http://2010.igem.org/wiki/index.php?title=Team:Newcastle/Spider_silk&diff=8293&oldid=prev
RachelBoyd at 11:33, 12 June 2010
2010-06-12T11:33:10Z
<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Newcastle/mainbanner}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Newcastle/mainbanner}}</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">=Spider Silk=</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Background Info==</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">|'''''Spider Silk'''''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">|- </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|'''World’s strongest spider silk''' comes from species of the genus ''Nephila''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|'''World’s strongest spider silk''' comes from species of the genus ''Nephila''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|- </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|- </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|[[Image:Spider Web.jpg]][[Image:Golden orb web.jpg]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|[[Image:Spider Web.jpg]][[Image:Golden orb web.jpg]]</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">|}</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Major Ampulate Spidroin Proteins==</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{|</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|'''Spider silk''' is comparable in strength to carbon fibres</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|'''Spider silk''' is comparable in strength to carbon fibres</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|''B.subtilis'' potential host as simple secretion system compared to yeast. Secretion has advantages over expression in ''E.coli'' however; insufficient proportion of protein was secreted by yeast.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|''B.subtilis'' potential host as simple secretion system compared to yeast. Secretion has advantages over expression in ''E.coli'' however; insufficient proportion of protein was secreted by yeast.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>| '''Fahnestock, S. R., Yao, Z., & Bedzyk, L. a. (2000). Microbial production of spider silk <del class="diffchange diffchange-inline">proteins</del>. Journal of biotechnology, 74(2), 105-19. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/11763501.'''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>| '''Fahnestock, S. R., Yao, Z., & Bedzyk, L. a. (2000). Microbial production of spider silk <ins class="diffchange diffchange-inline">pr}oteins</ins>. Journal of biotechnology, 74(2), 105-19. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/11763501.'''</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|}</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">==Self Assembly and pH==</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">{|</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|Self assembly of Spider silk proteins is controlled by a pH sensitive relay.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|A drop to pH 6.3 and shearing forces are believes to result in the hard form of spider silk.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|- </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|Miniature spidroins (a few repeats 4repCT) form metre long fibres irrespective of pH. </ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|Introduction of N-terminal domain from MASP1 (''Euprosthenops australis'')</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|to mini spidroins allows self assembly at pH 6.3 and delays aggregation above pH7. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|- </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|Relay- like mechanism N terminal regulates assembly. pH 8 NT-mini spidroins for long firbes over days at pH 6 they form rapidly.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|C-terminal domain promotes Silk formation by ordering the repetitive segments. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|'''Askarieh, G. et al., 2010. Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. Nature, 465(7295), 236-238. Available at: http://www.nature.com/doifinder/10.1038/nature08962.'''</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|}</ins></div></td></tr>
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RachelBoyd
http://2010.igem.org/wiki/index.php?title=Team:Newcastle/Spider_silk&diff=8205&oldid=prev
RachelBoyd at 21:02, 11 June 2010
2010-06-11T21:02:56Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|Its impressive web can be up to 6metres long and 2metres wide and last several years.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|Its impressive web can be up to 6metres long and 2metres wide and last several years.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|It has long been used by man; tribal people wrapped around <del class="diffchange diffchange-inline">a stick </del>to catch fish. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|It has long been used by man; tribal people wrapped <ins class="diffchange diffchange-inline">it </ins>around <ins class="diffchange diffchange-inline">sticks </ins>to catch fish. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|In a modern setting its uses are becoming more and more valuable from parachutes to bullet proof vests and in this</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|In a modern setting its uses are becoming more and more valuable from parachutes to bullet proof vests and in this</div></td></tr>
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RachelBoyd
http://2010.igem.org/wiki/index.php?title=Team:Newcastle/Spider_silk&diff=8202&oldid=prev
RachelBoyd at 21:01, 11 June 2010
2010-06-11T21:01:05Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 21:01, 11 June 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|'''''Spider Silk'''''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|'''''Spider Silk'''''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">[[Image:Spider Web.jpg]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|- </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|'''World’s strongest spider silk''' comes from species of the genus ''Nephila''</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|- </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|- </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|Spider silk is comparable in strength to carbon fibres</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|<ins class="diffchange diffchange-inline">(The golden orb spider: the largest spider in this genus is ''Nephila maculata'') </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|Its impressive web can be up to 6metres long and 2metres wide and last several years.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|It has long been used by man; tribal people wrapped around a stick to catch fish. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|In a modern setting its uses are becoming more and more valuable from parachutes to bullet proof vests and in this</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|project as measure to help fix cracks in concrete. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|http://www.naturia.per.sg/buloh/inverts/nephila.htm</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|[[Image:Spider Web.jpg]][[Image:Golden orb web.jpg]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|'''</ins>Spider silk<ins class="diffchange diffchange-inline">''' </ins>is comparable in strength to carbon fibres</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|Highly structured at the nanometre scale – not good for synthetic materials </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|Highly structured at the nanometre scale – not good for synthetic materials </div></td></tr>
</table>
RachelBoyd
http://2010.igem.org/wiki/index.php?title=Team:Newcastle/Spider_silk&diff=5756&oldid=prev
RachelBoyd: New page: {{Team:Newcastle/mainbanner}} {| |- |'''''Spider Silk''''' Image:Spider Web.jpg |- |Spider silk is comparable in strength to carbon fibres |- |Highly structured at the nanometre scale...
2010-05-31T10:39:58Z
<p>New page: {{Team:Newcastle/mainbanner}} {| |- |'''''Spider Silk''''' <a href="/File:Spider_Web.jpg" title="File:Spider Web.jpg">Image:Spider Web.jpg</a> |- |Spider silk is comparable in strength to carbon fibres |- |Highly structured at the nanometre scale...</p>
<p><b>New page</b></p><div>{{Team:Newcastle/mainbanner}}<br />
{|<br />
|-<br />
|'''''Spider Silk'''''<br />
[[Image:Spider Web.jpg]]<br />
|- <br />
|Spider silk is comparable in strength to carbon fibres<br />
|-<br />
|Highly structured at the nanometre scale – not good for synthetic materials <br />
|-<br />
|Repetitive structures- GXG motif<br />
|-<br />
|Glycine rich segments – hard and soft segments alternating<br />
|-<br />
| '''Hard'''= hydrogen bonding cross-linked crystallites (polyalanine) forming an amorphic beta sheet structure, <br />
|-<br />
|'''Soft'''= flexibility (Glycine rich) <br />
|- <br />
|Major protein from ''Nephila clavipes'' – MaSP1 tandem variants of <br />
|-<br />
|A GQG GYG GLG SQG A GRG GLG GQG A GA6GGx<br />
|-<br />
|MaSP2 also has a repetitive structure – difference soft segment contains proline containing pentamers: The consensus repeat is _GPGGY GPGQQ.3GPSGPGS A8. Similar structure to Elastin – elastic properties of drag-line by the folding of pentamer structure. <br />
|-<br />
|In the spider – silk in 3 phases<br />
|-<br />
|1) Extremely viscous (withstand shear forces inside spider),<br />
|-<br />
|2) Liquid crystallite lower viscosity (near exit duct/glycine rich may be involved),<br />
|- <br />
|3) Insoluble fibre (result of dehydration and drawing). <br />
|- <br />
|'''MaSP1 and MaSP2''' – Drag line <br />
|-<br />
|'''MaSP1'''-Auxilary <br />
|-<br />
|'''MaSP2'''- Glue silk only<br />
|-<br />
|'''Neither'''- Cocoon silk<br />
|-<br />
|Super contraction associated with pentamer motif when wet: low visco-elasticity <br />
|-<br />
|Mimic natural proteins or simplify – Mimic structural significance still uncertain for some sequences<br />
|- <br />
|DPB1- Optimised for ''B.subtilis'' <br />
|-<br />
|''B.subtilis'' potential host as simple secretion system compared to yeast. Secretion has advantages over expression in ''E.coli'' however; insufficient proportion of protein was secreted by yeast.<br />
|-<br />
| '''Fahnestock, S. R., Yao, Z., & Bedzyk, L. a. (2000). Microbial production of spider silk proteins. Journal of biotechnology, 74(2), 105-19. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/11763501.'''</div>
RachelBoyd