Team:Newcastle/Spider silk

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|'''''Spider Silk'''''
|'''''Spider Silk'''''
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[[Image:Spider Web.jpg]]
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|'''World’s strongest spider silk''' comes from species of the genus ''Nephila''
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|Spider silk is comparable in strength to carbon fibres
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|(The golden orb spider: the largest spider in this genus is ''Nephila maculata'')
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|Its impressive web can be up to 6metres long and 2metres wide and last several years.
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|It has long been used by man; tribal people wrapped around a stick to catch fish.
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|In a modern setting its uses are becoming more and more valuable from parachutes to bullet proof vests and in this
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|project as measure to help fix cracks in concrete.
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|http://www.naturia.per.sg/buloh/inverts/nephila.htm
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|[[Image:Spider Web.jpg]][[Image:Golden orb web.jpg]]
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|'''Spider silk''' is comparable in strength to carbon fibres
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|Highly structured at the nanometre scale – not good for synthetic materials  
|Highly structured at the nanometre scale – not good for synthetic materials  

Revision as of 21:01, 11 June 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map
Spider Silk
World’s strongest spider silk comes from species of the genus Nephila
(The golden orb spider: the largest spider in this genus is Nephila maculata)
Its impressive web can be up to 6metres long and 2metres wide and last several years.
It has long been used by man; tribal people wrapped around a stick to catch fish.
In a modern setting its uses are becoming more and more valuable from parachutes to bullet proof vests and in this
project as measure to help fix cracks in concrete.
http://www.naturia.per.sg/buloh/inverts/nephila.htm
Spider Web.jpgGolden orb web.jpg
Spider silk is comparable in strength to carbon fibres
Highly structured at the nanometre scale – not good for synthetic materials
Repetitive structures- GXG motif
Glycine rich segments – hard and soft segments alternating
Hard= hydrogen bonding cross-linked crystallites (polyalanine) forming an amorphic beta sheet structure,
Soft= flexibility (Glycine rich)
Major protein from Nephila clavipes – MaSP1 tandem variants of
A GQG GYG GLG SQG A GRG GLG GQG A GA6GGx
MaSP2 also has a repetitive structure – difference soft segment contains proline containing pentamers: The consensus repeat is _GPGGY GPGQQ.3GPSGPGS A8. Similar structure to Elastin – elastic properties of drag-line by the folding of pentamer structure.
In the spider – silk in 3 phases
1) Extremely viscous (withstand shear forces inside spider),
2) Liquid crystallite lower viscosity (near exit duct/glycine rich may be involved),
3) Insoluble fibre (result of dehydration and drawing).
MaSP1 and MaSP2 – Drag line
MaSP1-Auxilary
MaSP2- Glue silk only
Neither- Cocoon silk
Super contraction associated with pentamer motif when wet: low visco-elasticity
Mimic natural proteins or simplify – Mimic structural significance still uncertain for some sequences
DPB1- Optimised for B.subtilis
B.subtilis potential host as simple secretion system compared to yeast. Secretion has advantages over expression in E.coli however; insufficient proportion of protein was secreted by yeast.
Fahnestock, S. R., Yao, Z., & Bedzyk, L. a. (2000). Microbial production of spider silk proteins. Journal of biotechnology, 74(2), 105-19. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/11763501.