Team:Newcastle/Slide Preparation

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1. Grow overnight culture in 5ml of LB broth at 37C
2. Dilute the overnight culture to a ratio of 1:100 and incubate at 37C for 1 hour
3. Set up the following broths with different concentrations of IPTG in 5ml of LB broth

• 2mM IPTG
• 0.2mM IPTG
• 0.02mM IPTG
• Broth only