Team:Newcastle/Scanning EM

From 2010.igem.org

Revision as of 22:02, 27 October 2010 by Swoodhouse (Talk | contribs)

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Contents

Scanning EM

This is a standard method for Scanning Electron Microscopy. Specimens vary greatly and the final protocol should be decided by referring to text books and publications.

Fixation

Fix in 2% Gluteraldehyde in Sorensons Phosphate Buffer overnight (minimum).

Rinse in Sorensons Phosphate Buffer 2x 15 mins.

Dehydration

25% ethanol: 30 mins. 50% ethanol: 30 mins. 75% ethanol: 30 mins. 100% ethanol: 1 hr. 100% ethanol: 1 hr.

Once in 100% ethanol, samples are dried using a Baltec Critical Point Dryer.

Mounting

Mount of an aluminium stub with Achesons Silver ElectroDag.

Gold coating

Coat with 15nm of gold using a Polaron SEM Coating Unit.

Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon