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Scanning EM=

This is a standard method for Scanning Electro Microscopy. Specimens vary greatly and the final protocol should be decided by referring to text books and publications.

Fixation

Fix in 2% Gluteraldehyde in Sorensons Phosphate Buffer overnight (minimum). Rinse in Sorensons Phosphate Buffer 2x 15 mins.

Dehydration

25% ethanol: 30 mins. 50% ethanol: 30 mins. 75% ethanol: 30 mins. 100% ethanol: 1 hr. 100% ethanol: 1 hr.

Once in