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Team:Newcastle/Meetings/9 September 2010
From 2010.igem.org
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==Modelling== | ==Modelling== | ||
| - | Metabolic Flux Balance Analysis - alter enzyme levels to produce different outcomes | + | Metabolic Flux Balance Analysis - Show the judges what happen when we alter the enzyme levels to produce different outcomes. |
==Lab feedback== | ==Lab feedback== | ||
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* Subtilin immunity - Same as ''rocF'' | * Subtilin immunity - Same as ''rocF'' | ||
* ''yneA'' - Transforming into ''Bacillus subtilis'' 168 strains works and we have filamentous cells. However still not able to integrate ''yneA'' into Pmutin strains. Could use a plasmid that have already contain the IPTG system. For characterization, we could either do a titration with IPTG together with time lapse microscopy to measure the length of the cells. | * ''yneA'' - Transforming into ''Bacillus subtilis'' 168 strains works and we have filamentous cells. However still not able to integrate ''yneA'' into Pmutin strains. Could use a plasmid that have already contain the IPTG system. For characterization, we could either do a titration with IPTG together with time lapse microscopy to measure the length of the cells. | ||
| + | *Arabinose promoter - Insert the promoter in front of GFP and characterize with the addition of arabinose. | ||
==Concrete== | ==Concrete== | ||
| - | + | * Use different bacteria to seal up the cracks. | |
| - | * Use | + | * Preincubate the concrete in media. |
| - | * | + | |
==Wiki== | ==Wiki== | ||
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ESTA must be completed (if required) '''ASAP'''. | ESTA must be completed (if required) '''ASAP'''. | ||
| - | == | + | ==T-shirt== |
| - | + | Upload the t-shirt files into the dropbox. | |
| - | + | ||
| - | + | ||
| - | + | ||
==Action points== | ==Action points== | ||
| - | * ''' | + | * '''Neil and Jen''' - To register the team. |
| - | * ''' | + | * '''Alan''' - To email Jen about the IPTG plasmid and the letters for the sucrose plates. |
| - | + | * '''Harsh''' - To crack the concrete into smaller pieces. | |
| - | * '''Harsh''' - | + | * '''Wendy''' - To talk to the EM people. |
| - | * ''' | + | |
| - | + | ||
==Item(s) for next agenda== | ==Item(s) for next agenda== | ||
Revision as of 20:40, 13 September 2010
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Contents |
Roll calls
- Apologies: Phil and Deena
- Absence: Colin Harwood and Colin Davie
Modelling
Metabolic Flux Balance Analysis - Show the judges what happen when we alter the enzyme levels to produce different outcomes.
Lab feedback
- Gibson cloning - Joining of 2 fragments together works, but could not be ligated into the vector. Need more time to perfect the technique.
- Subtilin immunity - Same as rocF
- yneA - Transforming into Bacillus subtilis 168 strains works and we have filamentous cells. However still not able to integrate yneA into Pmutin strains. Could use a plasmid that have already contain the IPTG system. For characterization, we could either do a titration with IPTG together with time lapse microscopy to measure the length of the cells.
- Arabinose promoter - Insert the promoter in front of GFP and characterize with the addition of arabinose.
Concrete
- Use different bacteria to seal up the cracks.
- Preincubate the concrete in media.
Wiki
Lab book is up to date.
Visas
ESTA must be completed (if required) ASAP.
T-shirt
Upload the t-shirt files into the dropbox.
Action points
- Neil and Jen - To register the team.
- Alan - To email Jen about the IPTG plasmid and the letters for the sucrose plates.
- Harsh - To crack the concrete into smaller pieces.
- Wendy - To talk to the EM people.
Item(s) for next agenda
- Go through the presentation slides and refine. Cut down the number of slides so that it fits the 20 minutes time limit
Next meeting
- Chair: Harsh, Minutes: Younus, Computer: N/A
- Wednesday afternoon, 3pm, CBCB.
   
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