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Team:Newcastle/Meetings/7 July 2010
From 2010.igem.org
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==Roll call== | ==Roll call== | ||
Everyone here except Racheal, Colin and Jennifer. | Everyone here except Racheal, Colin and Jennifer. | ||
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==Research feedback== | ==Research feedback== | ||
* Swarming function. ''sfP'' and ''swrA'' genes needed from 3610 marburg strain. Swarming depends on the two-component system of ''comP'' and ''comA'' which is activated by ''comX'' and ''csF''. ComA then activate srfABCD, leadint to surfactin production. ''comS'' will also be activated which is for competence. Surfactin may break the surface tension to allow movement. Frame shift mutation is the ''sfP'' gene which is needed for the post translational modification of the surfactin. Mutation in the ''swrA'' too, which is needed for the flagella biosynthesis. Surfactin also an amp, however the immunity gene is found in the 168 strain. | * Swarming function. ''sfP'' and ''swrA'' genes needed from 3610 marburg strain. Swarming depends on the two-component system of ''comP'' and ''comA'' which is activated by ''comX'' and ''csF''. ComA then activate srfABCD, leadint to surfactin production. ''comS'' will also be activated which is for competence. Surfactin may break the surface tension to allow movement. Frame shift mutation is the ''sfP'' gene which is needed for the post translational modification of the surfactin. Mutation in the ''swrA'' too, which is needed for the flagella biosynthesis. Surfactin also an amp, however the immunity gene is found in the 168 strain. | ||
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| + | ==Lab feedback== | ||
| + | * Arginine tests - Harsh and Alan. No pH change observed. Use a minimal medium, as other nitrogen source might be present in LB. | ||
| + | * Urea tests - Harsh and Alan. Pink colour change observed. Urea being breakdown by ''B subtilis''. | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Revision as of 12:30, 12 July 2010
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Roll call
Everyone here except Racheal, Colin and Jennifer.
Research feedback
- Swarming function. sfP and swrA genes needed from 3610 marburg strain. Swarming depends on the two-component system of comP and comA which is activated by comX and csF. ComA then activate srfABCD, leadint to surfactin production. comS will also be activated which is for competence. Surfactin may break the surface tension to allow movement. Frame shift mutation is the sfP gene which is needed for the post translational modification of the surfactin. Mutation in the swrA too, which is needed for the flagella biosynthesis. Surfactin also an amp, however the immunity gene is found in the 168 strain.
Lab feedback
- Arginine tests - Harsh and Alan. No pH change observed. Use a minimal medium, as other nitrogen source might be present in LB.
- Urea tests - Harsh and Alan. Pink colour change observed. Urea being breakdown by B subtilis.
   
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