Team:Newcastle/Meetings/6 October 2010

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Contents

6 October 2010 Meeting

Roll call

  • Apologies: Colin Harwood and Jem

Presentation

Introduction

  • Mention why do we use this bug and that it is able to live in high pH
  • Have to menetion that we all biobrick have been modelled by SBML and copasi

Bacilla Filla slide

  • The solution words to be smaller than the logo
  • Logo have to be the biggest!
  • Solution at the top of the page with a small logo below it then zoom into the logo

Technical review slide

  • Show the link between the genes
  • Some of the summary slide's information to go here, example: swim down the cracks, the tent, spray on, etc.
  • A single shot overview then to the biobricks
  • SAY: This is a crack, and here is our bug on the surface of the crack, etc
  • Label the pictures!
  • Animation for the overview, ie, the background to remain the same

Swarming slide

  • Change the flagella of the bug
  • Zoom into the plates

Subtilin slide

  • Show subtilin only when the bug is at the end of the crack
  • Fill up the crack with bugs
  • Describle the subtilin signalling system
  • Change the pveg promoter to the oxygen sensitive promoter

Subtilin immunity slide

  • SAY: Subtilin immunity ablready in the registry, that is why we use it
  • Mention what the individual CDS do
  • Green tick for those that we did and a red cross for those that we did not

Workflow slide

  • Change the word interesting to valuable to all synthetic biologist
  • Cut down on the script
  • Have to make it fit to the presentation
  • Integration of lots of data that is why we use e-science

YneA slide

  • Put filamentous cells into the pictures
  • Metabolic pathway picture and link it

Glue slide

  • The iGEM plates to go onto it

Kill switch

  • Can bin it
  • Move to the ethic page

Ethic slide

  • Move it to the front after the introduction and also mention in the summary
  • What is the danger and how we make it safe

Others

  • Show that the bug is able to grow in concrete

Urease slide

  • Have to show that calcium carbonate is filling up the cracks
  • Have to say that we are better than the rest
  • Highlight the advantage of ours and the disadvantage of others
  • Mention SR1

Lab feedback

Filamentous cells

  • Redo the microscopic
  • Correlate between the expression of GFP and the length

SR1

  • We have colonies

Scanning electron microscopy

  • The concrete samples are already with the EM unit

Agenda

  • To reedit the agenda

Action points

  • Rachel and Phil: Characterisation data for filamentous cells brick needs to be put on the wiki and the parts registry.
  • Everyone to do up the presentation slides first.

Next meeting

Wed 4pm.

  • Chair: Steven
  • Computer: Alan
  • Minutes: Deena