Team:Newcastle/Meetings/4 June 2010


Revision as of 20:54, 6 June 2010 by Yessa (Talk | contribs)
Informal Meeting 04.06.2010
Urease tutorial-recap
Accessory genes and nickel incorporation necessary more complicated than we thought.
Software engineering cycle
Requirements -> specification-> design-> implementation -> testing -> maintenance back to the requirements
Specification more detail on the requirements- literature
Urease calcium carbonate, optimise for Bacillis
Increase expression- what we thought!
Biosynthesis of active BS in the Absence of Known Urease Accessory Proteins: paper
Accessory genes required!
Look over papers from tutorial
Sporosarcina pasteurii
Biobrick site symbols
Promoter-ribosome- binding- coding sequence –ribosome binding site-coding-ribosome binding coding- terminator sequences ...and spacers
ncbi – find the genome – can see the genes
Implementation using sequencher
Find the sequence of part
Assemble parts
Biobrick friendly restriction sites
Genbank/embl file format
Implementation altered by cloning strategy
Biobricks RFC- request for comments -duplo and lego don’t fit together
AP Rachel: video for lego pipette
1st lab week Others modelling Filamentous and spidersilk killswitch
2nd lab week Others Modelling glue and lysis quorum sensing
Urease both


  • Group 1 starts 14th June: SW, D.Y AK, (HS); working on Biobrick 2 & 5 (End of crack & signalling system; Glue & lysis)
  • Group 2 starts 21st June: JS, RB, RC, PH, (YE); working on BioBrick 3 & 4 (Non-target-environment kill switch; Glue & lysis)
  • All team members working on BioBrick 1 (CaCO3/Urease).
  • RC working on BioBrick 6 (sequential programming)

  • Team members not in the lab training will be working on modelling?
  • HS and YE will unfortunately be away for both lab training sessions, hence the brackets