Team:Newcastle/Meetings/26 May 2010

From 2010.igem.org

(Difference between revisions)
(Formal Meeting 26th May 2010)
(Formal Meeting 26th May 2010)
Line 20: Line 20:
   | 4. Research Feedback
   | 4. Research Feedback
|-
|-
-
         |1. Nutrient
+
         |1. Bibrick 1 (CaCO3/ Urease)
-
Using a glucose and starch concentration gradient. Uses the catabolic repression system to allow glue production. Have to test and find suitable starch and chemoattractant!!!!!!!!
+
Biobrick tutorial taught last to last week by Anil on Urease. Urease has a complicated genetic circuit. Should start forming questions which would help in building the egnetic circuit of the biobrick.
|-
|-
         |2. Sporulation, germination and secretion control
         |2. Sporulation, germination and secretion control

Revision as of 09:20, 29 May 2010

Formal Meeting 26th May 2010

Minutes for formal meeting 1st April 2010
* Room: Claremont 922; Start: 3 pm
* Chair: Rachel, Minutes: Harsh, Computer: Rachel
1. Roll call and apologies
Apologies - Steven, Younus, Da, Alan, Phil
2. Approval of agenda and minutes
Approved
3. Brief summary of previous meeting
4. Research Feedback
1. Bibrick 1 (CaCO3/ Urease)

Biobrick tutorial taught last to last week by Anil on Urease. Urease has a complicated genetic circuit. Should start forming questions which would help in building the egnetic circuit of the biobrick.

2. Sporulation, germination and secretion control

AbrB gene maybe a suitable gene to repress sporulation by overexpressing it. Can also activate antimicrobial peptides like TasA. Immune the concrete from future attack by concrete destroying bacteria? Need to find what other genes that are involve in the pathway of AbrB.

3. Quorum Sensing

Number of competence cells not an issue. Sin operone to control a lot of pathway.

4. Other points

A. FtsZ knockout to allow filamentous growth, thereby forming a mesh with glue either secreted out or remains in the cells until lysis. B. Is spider silk able to resist the alkali conditions? C. Is it possible to produce spider silk in Bacillus?

5. Voigt paper feedback

Salmonella to secrete spider silk contained in SPI through T3SS. SPI to contain ADF1, ADF2 and ADF3 genes. Does Bacillus have a T3SS? Can we allow proteins to fold in the cells then induce lysis? To check out Bacillus secretion system.

6. Visa/Passport update

Harsh to apply on May, June period

7. Travel plans

Too early to book flights and hotels. May have other members joining.

8. Tutorial

Next tutorial to be on the week after

9. Flow Chart

Media:Flowchart_overview-1-.ppt‎ A. Need to be more in depth. ie. The problems, what the bacteria need to do to achieve the goal and the genes that are needed and why. B. Another flow chart to show the different stituation and how the bacteria response to it.

10. Sponser on T-shirt (Janetta)

To contact sponser next week

11. Other business

A. Foxit not working

12. Action points

1. To draft email to Colin and Chris and show it to instructors first(everyone) 2. To continue to develop flow chart(everyone) 3. Tutorial on Wiki

14. Next meeting 11am on 070410 for informal meeting in Walton library and 3pm on 070410 for formal meeting in CBCB
        1. Chair-Richard 
        2. Minutes-Racheal
        3. Computer-Jenetta