Team:Newcastle/Meetings/26 July 2010

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(New page: {{Team:Newcastle/mainbanner}} ==Roll call== Everyone here except Colin Harwood, Colin Davie and Wendy Smith ==Research feedback== # Levan glue - Levan requires sucrose to work * Test how...)
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{{Team:Newcastle/mainbanner}}
{{Team:Newcastle/mainbanner}}
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==Roll call==
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==Formal Meeting - 26th July 2010==
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Everyone here except Colin Harwood, Colin Davie and Wendy Smith
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==Research feedback==
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*[[Team:Newcastle/Agendas/26_July_2010| Agenda]]
 +
====Roll call====
 +
*Everyone present except Da, Prof. Colin Harwood, Dr Colin Davie and Dr Wendy Smith
 +
 
 +
====Research feedback====
# Levan glue - Levan requires sucrose to work
# Levan glue - Levan requires sucrose to work
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* Test how much sucrose is needed - Phil
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#* Test how much sucrose is needed - Phil
# Kill switch - Use of ''mazE'' and ''mazF''
# Kill switch - Use of ''mazE'' and ''mazF''
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* How much sucrose is needed for it to work?
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#* How much sucrose is needed for it to work?
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* How fast does the bug use up all the sucrose?
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#* How fast does the bug use up all the sucrose?
# Stochastic switch - Use of ''hinC'' as the invertase
# Stochastic switch - Use of ''hinC'' as the invertase
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* Matt to work with Phil to work out the probability of the switching
+
#* Matt to work with Phil to work out the probability of the switching
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* Have to work out what is wrong with the iGEM 2009 stochastic switch first
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#* Have to work out what is wrong with the iGEM 2009 stochastic switch first
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# Gibson protocol
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==Lab feedback==
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#* ''rocF'' to require 6 pair of primers, therefore try doing up 2 fragments first
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Phil and Younus - LacI Transformation, MiniPrep and Restriction digests
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# Protocol for ''yneA''
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* Transformants with LacI and RFP in it. Not sure how to test if LacI is in the plasmid
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#* Do up the characterization separately, one bacteria to contain ''yneA'' to show filamentous, the other to have the ''lacI''
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* Whether gel, transformation worked, Miniprep did not work, plasmid moving at the wrong band (too many bands)
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#* Could have nice photo with the different morphology on agar
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Arrangement in lab - yneA (synthesizing), SR1 (Sending to synthesize), Swarming,
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#* Do not shine UV light on during the gel extraction step to avoid DNA mutation
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Have Bacillus parts in one place (ask Wendy)
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#* Make up lots of DNA to allow better transformation
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==iGEM UK get together==
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* Starts at 12.30 on the 200710 and ends at 12.30 on 210710. Each team to give a 20 minutes talk, all teams from UK except Imperial are coming, 2 slides for each brick. Team building exercise. Dinner at La Tasca at Quayside. Next morning: talks. Matthew Robinson talk.
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* Visitors staying in university halls
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==Logo and T-shirts==
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* Have all logos on t-shirt
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* Bacilla filla words on top of logo
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==Visas==
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* Harsh to travel to London on 160710.
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==Travel plans==
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====Lab feedback====
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* Book flights from 041110 to the 081110
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# Arginine test - Alan and Harsh
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* Accommodation open for booking
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#* Test worked fine.
 +
#* Could refine the test by using universal liquid pH indicator or to use the pH meter
 +
# DNA extraction - Harsh and Racheal
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#* Could only confirm the result on 27.07.2010
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==Funding==
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====Wiki====
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* School of Biomedical Sciences
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* Not able to do the drop down menu with the shadow test, therefore could try using htm5
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* Mr. Gene might be able to fund us because we display their logo on wiki
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* Horizontal strip to change to purple
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==Other business==
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====Visas====
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* If start later, email everyone first.
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* Deena to travel to London on 12.08.2010
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==Action points==
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====Action point====
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* Write thank you letter to David Parker
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* Talk to Louela - Phil and Harsh
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* CEG logo on website
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* Email to iGEM HQ to get stickers and badges - Harsh
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* Talk to Lula about funding
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* Hyperlink the protocol page to the lab notebook
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* Get back to Centre of Life person
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* Lab wiki checker - Alan and Harsh
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* Contact Megan Lazara for iGEM badges and stickers
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* Send reminder to Jen to add Jane to the mailing list - Jannetta
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* Phil to send an email to Jill Green about Get Together
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* Put CBCB and Biomedical school logo onto the wiki - Janetta
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* Legends on figures(table caption goes on top, picture captions bottom), write on, description
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* Write to Chris Voigt to get the source code - Steven
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* Create a page for cloning strategy like last year's
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* Tutorial for Inkscape - Jannetta
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* Twitter feed
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* To check if the extra USA visa immigration form is valid for 2 years - Jen
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* Jannetta to change the gliffs
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* Remind Neil about the MrGene logo - Steven
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* What to do with yneA when it comes back
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* Plan every step before going into lab
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* Fill in wiki everyday for lab work
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* Put down where we get protocols from
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* Have a protocol page and link all experiments to that page
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* Put Jane Calvert on iGEM mailing list
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* Put all school logos on website
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* Fill immigration form about 2 weeks before jamboree
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==Next meeting==
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====Next meeting====
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* Steven is chair, Deena is computer, Alan is minutes
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* Chair: Phil, Computer: Alan, Minutes: Rachel
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* Friday afternoon, 4pm.
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* Next Wednesday, 3pm
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 01:36, 26 October 2010

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Contents

Formal Meeting - 26th July 2010

Roll call

  • Everyone present except Da, Prof. Colin Harwood, Dr Colin Davie and Dr Wendy Smith

Research feedback

  1. Levan glue - Levan requires sucrose to work
    • Test how much sucrose is needed - Phil
  2. Kill switch - Use of mazE and mazF
    • How much sucrose is needed for it to work?
    • How fast does the bug use up all the sucrose?
  3. Stochastic switch - Use of hinC as the invertase
    • Matt to work with Phil to work out the probability of the switching
    • Have to work out what is wrong with the iGEM 2009 stochastic switch first
  4. Gibson protocol
    • rocF to require 6 pair of primers, therefore try doing up 2 fragments first
  5. Protocol for yneA
    • Do up the characterization separately, one bacteria to contain yneA to show filamentous, the other to have the lacI
    • Could have nice photo with the different morphology on agar
    • Do not shine UV light on during the gel extraction step to avoid DNA mutation
    • Make up lots of DNA to allow better transformation

Lab feedback

  1. Arginine test - Alan and Harsh
    • Test worked fine.
    • Could refine the test by using universal liquid pH indicator or to use the pH meter
  2. DNA extraction - Harsh and Racheal
    • Could only confirm the result on 27.07.2010

Wiki

  • Not able to do the drop down menu with the shadow test, therefore could try using htm5
  • Horizontal strip to change to purple

Visas

  • Deena to travel to London on 12.08.2010

Action point

  • Talk to Louela - Phil and Harsh
  • Email to iGEM HQ to get stickers and badges - Harsh
  • Hyperlink the protocol page to the lab notebook
  • Lab wiki checker - Alan and Harsh
  • Send reminder to Jen to add Jane to the mailing list - Jannetta
  • Put CBCB and Biomedical school logo onto the wiki - Janetta
  • Write to Chris Voigt to get the source code - Steven
  • Tutorial for Inkscape - Jannetta
  • To check if the extra USA visa immigration form is valid for 2 years - Jen
  • Remind Neil about the MrGene logo - Steven

Next meeting

  • Chair: Phil, Computer: Alan, Minutes: Rachel
  • Next Wednesday, 3pm
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