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Team:Newcastle/Meetings/1 July 2010
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| - | == | + | {{Team:Newcastle/mainbanner}} |
| - | + | ==Formal Meeting - 1st July 2010== | |
| - | == | + | *[[Team:Newcastle/Agendas/1_July_2010#Formal_Meeting_-_1st_July_2010| Agenda]] |
| - | + | ====Roll call==== | |
| - | + | Everyone here except Phil and Younus who are in the lab. Arrived 10 mins later. | |
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| - | ==Lab feedback== | + | ====Action points from last week==== |
| - | Arginine and urease tests - Harsh and Alan. The experiment has been designed, waiting for the materials. In the lab at 4. | + | * Wiki changes have been made. |
| - | Urea media, grown overnight, If the urea is used, there will be a colour change. | + | * Letter to Andrew Young is not finished - someone needs to be in charge of this. |
| + | * Civil engineer funding is not done yet. | ||
| + | * Flights: plan is to get there on Thursday about 4-5 p.m. | ||
| + | |||
| + | ====Research feedback - end of crack signalling, quorum sensing==== | ||
| + | * Oxygen limitation triggers subtilin production, ''ResD'' and ''E''. | ||
| + | * Need subtilin immunity brick too ''spaIFEG''. | ||
| + | * Subtilin receptor was done in 2008 by Newcastle ''spaRK''. | ||
| + | * Want it to be triggered by both oxygen limitation and by subtilin. Oxygen to trigger the production at the end of the crack, subtilin to propagate the signal. | ||
| + | * We need to get the strain with these genes. ATCC6633. | ||
| + | |||
| + | ====Lab feedback==== | ||
| + | *Arginine and urease tests - Harsh and Alan. The experiment has been designed, waiting for the materials. In the lab at 4 pm. | ||
| + | *Urea media, grown overnight, If the urea is used, there will be a colour change. | ||
| + | |||
| + | *Arginine test. Bug in LB, supplement with arginine. If arginine is used, pH increase. | ||
| + | |||
| + | *Phil and Younus, transforming ''E. coli'' dh5alpha with the vector that we will be using for lacI. Plasmid extraction tomorrow. | ||
| + | |||
| + | ====Logo and T-shirts==== | ||
| + | * Finalised. | ||
| + | * University logo must be correct - take it from the University website, not from anywhere else. Fill in the form to get the high resolution image. | ||
| + | * CBCB logo needs to be added. Ask Jeff or Joanna. | ||
| + | |||
| + | ====Visas==== | ||
| + | * Younus still hasn't filled in the visa-waiver form. | ||
| + | |||
| + | ====Funding==== | ||
| + | * Andrew Young letter needs to be finished and checked tomorrow. | ||
| + | |||
| + | ====Timeline==== | ||
| + | * Could be made more detailed now. | ||
| + | * Might need another brick, for motility or swarming. Definitely do not want biofilm formation. | ||
| + | * Spider silk next? It's on our logo so we should do it. | ||
| + | |||
| + | ====Action points==== | ||
| + | * Phil: talk to Dr Aldridge about glue. How easy would it be to design a glue BioBrick? | ||
| + | * All: Andrew Young letter needs to be finished and checked tomorrow. | ||
| + | |||
| + | ====Next meeting==== | ||
| + | *Next Wednesday, 3 p.m. | ||
| + | *Chair: Harsh, Minutes: Alan, Computer: Phil | ||
| + | {{Team:Newcastle/footer}} | ||
Latest revision as of 01:38, 26 October 2010
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Contents |
Formal Meeting - 1st July 2010
Roll call
Everyone here except Phil and Younus who are in the lab. Arrived 10 mins later.
Action points from last week
- Wiki changes have been made.
- Letter to Andrew Young is not finished - someone needs to be in charge of this.
- Civil engineer funding is not done yet.
- Flights: plan is to get there on Thursday about 4-5 p.m.
Research feedback - end of crack signalling, quorum sensing
- Oxygen limitation triggers subtilin production, ResD and E.
- Need subtilin immunity brick too spaIFEG.
- Subtilin receptor was done in 2008 by Newcastle spaRK.
- Want it to be triggered by both oxygen limitation and by subtilin. Oxygen to trigger the production at the end of the crack, subtilin to propagate the signal.
- We need to get the strain with these genes. ATCC6633.
Lab feedback
- Arginine and urease tests - Harsh and Alan. The experiment has been designed, waiting for the materials. In the lab at 4 pm.
- Urea media, grown overnight, If the urea is used, there will be a colour change.
- Arginine test. Bug in LB, supplement with arginine. If arginine is used, pH increase.
- Phil and Younus, transforming E. coli dh5alpha with the vector that we will be using for lacI. Plasmid extraction tomorrow.
Logo and T-shirts
- Finalised.
- University logo must be correct - take it from the University website, not from anywhere else. Fill in the form to get the high resolution image.
- CBCB logo needs to be added. Ask Jeff or Joanna.
Visas
- Younus still hasn't filled in the visa-waiver form.
Funding
- Andrew Young letter needs to be finished and checked tomorrow.
Timeline
- Could be made more detailed now.
- Might need another brick, for motility or swarming. Definitely do not want biofilm formation.
- Spider silk next? It's on our logo so we should do it.
Action points
- Phil: talk to Dr Aldridge about glue. How easy would it be to design a glue BioBrick?
- All: Andrew Young letter needs to be finished and checked tomorrow.
Next meeting
- Next Wednesday, 3 p.m.
- Chair: Harsh, Minutes: Alan, Computer: Phil
   
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