Team:Newcastle/Meetings/12 July 2010

Informal meeting - 12th July 2010

• Chair: Da, Minutes: Younus
• Walton Library [3.30 - 5.00pm]

Cloning Strategies

5 parts to a Cloning Strategy:

• The cloning strategies for each biobrick should contain the following sections in this order:
  1. Purpose and Justification
  2. Modelling
  3. Construction
  4. Cloning and Integration
  5. Testing and Characterising

Required Cloning Strategies

• There are 8 cloning strategies which are required:
  • SR1 - finished!
  • YneA - biobrick nearly coming back - our most important biobrick!
  • LacI - not sure how to test this biobrick
  • 2 for Swarming (sfp and swrA) - cloning strategies nearly finished
  • 2 for Subtilin (immunity and production) - cloning strategies nearly finished
  • RocF (need to use Gibson method) - not started

So only the cloning strategy for SR1 is finished. 7 more to go!

Allocation of team members to cloning strategies:

• YneA - Phil, Rach, Deena, Younus
• LacI - Phil, Younus, Rachel
• Swarming (2) - Harsh, Alan, Steve, Da
• Subtilin (2) - Harsh, Alan, Steve, Deena
• RocF - Harsh, Alan, Steve, Da

Wiki - Labbook

Everyone needs to carry out the following protocol for each day in the lab

1. Aim
2. Materials and Methods
3. Procedure/Protocol
4. Results
5. Conclusion

Relevant photographs should be added and labelled

General:

• Wiki checkers: Deena and Rachel (after altering/updating a page on the wiki, team members should send the links to the pages to the wiki checkers)

UK iGEM Gathering at Newcastle - 20th/21st July

Presentation:

Introduction

• Rach, Deena, Jannetta [2 mins]
  • Cover Page
  • Picture of us standing up
  • Introduction of our iGEM project - using BS to repair concrete

General Outlook

• Harsh, Da, Phil [5 mins]
  • Starting with bioreactor
  • Transformation into spores
  • Transportation
  • Spraying on building
  • Germination
  • sensing crack + non-environmental kill switch
  • swarming
  • end of crack signalling = QS
  • Filamentous cells
  • CaCO3

BioBrick Discussion

• Alan, Younus, Steve [8 mins altogether; 2 mins each]
  • Swarming
  • Subtillin
  • Filamentous cells - YneA
  • CaCO3 - Urease
  • LacI

Whats next...

• [5 mins remaining]
  • 'Save the babies!!!'

Minideadlines

1. Presentation slides to be e-mailed by tomorrow (Wed) 5 pm
2. Cloning Strategies - to be completed by Friday, 16th July 2010.
   • YneA
   • One sequence for sfp primers and coding sequence ready (Goksel)
   • Subtilin just immunity

Action Points

1. Finish wiki updates by Sunday 18th (everyone)
2. Team page on Wiki:
   • Add Colin Harwood, Colin Davies, Wendy Smith and Goksel Misrili to list of advisors
   • Deena, Phil and Younus to write about yourself + pictures