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Team:Newcastle/Meetings/12 July 2010
From 2010.igem.org
Chair: Da, Minutes: Younus, Comp
Contents |
Cloning Strategies
- SR1 - Finished
- RocF not started - need to use Gibson method;
- YneA
- 2 for Swarming (sfp and swrA)
- LacI
- 2 for Subtilin (Immunity and Production)
Only finished one, SR1. 7 more to go!
Next: swarming and subtilin nearly finished, YneA nearly coming back and LacI brick not sure how to test. Why is testing LacI hard?
- YneA - our most important BB - Phil, Rach, Deena, Younus
- Swarming (2) - Harsh, Alan, Steve, Da
- LacI - Phil, Younus, Rachel
- Subtilin (2) - Harsh, Alan, Steve, Deena
- RocF - Harsh, Alan, Steve, Da
5 parts to a Cloning Strategy:
- Purpose and justification
- Modelling
- Construction
- Cloning and Integration
- Testing and Characterising
Wiki
Labbook
- . Aim
- . Materials and Methods
- . Procedure/Protocol
- . Results
- . Conclusion
Everyone needs to carry out this protocol above Photographs add and label them In charge of wiki - Deena and Rachel (everyone everyday they have to send the links to both of them to check)
20th July - UK iGEM Gathering at Newcastle!
Presentation
Introduction - Rach, Deena, Jannetta
- Cover Page
- Picture of standing us up
- Introduction of our iGEM project - using BS to repair concrete (2 mins)
General Outlook (Harsh, Da, Phil) (5 mins):
- Starting with bioreactor
- Transformation into spores
- Transportation
- Spraying on building
- Germination
- sensing crack + non-environmental kill switch
- swarming
- end of crack signalling = QS
- Filamentous cells
- CaCO3
- Glue
- Kill Switch
BioBrick Discussion (Alan, Younus, Steve) (8 mins altogether; 2 mins each):
- Swarming
- Subtillin
- Filamentous cells - YneA
- CaCo3 - Urease
(5 mins remaining)
Whats next... 'Save the babies!!!'
Minideadlines:
- Cloning Strategies
- YnEA
- One sequence for sfp primers and coding sequence ready (Goksel)
- Subtilin just immunity
- Finish Wiki updates Sunday 18th
- Team page on Wiki:
- Add to list of advisors: Colin Harwood, Colin Daviy, Wendy Smith and Goksel Misrili
- Deena, Phil and Younus to write about yourself + pictures
