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From 2010.igem.org

Chair: Da, Minutes: Younus, Comp

1. . Cloning Strategies

• SR1 - Finished
• RocF not started - need to use Gibson method;
• YneA
• 2 for Swarming (sfp and swrA)
• LacI
• 2 for Subtilin (Immunity and Production)

Only finished one, SR1. 7 more to go!

Next: swarming and subtilin nearly finished, YneA nearly coming back and LacI brick not sure how to test. Why is testing LacI hard?

• YneA - our most important BB - Phil, Rach, Deena, Younus
• Swarming (2) - Harsh, Alan, Steve, Da
• LacI - Phil, Younus, Rachel
• Subtilin (2) - Harsh, Alan, Steve, Deena
• RocF - Harsh, Alan, Steve, Da

5 parts to a Cloning Strategy

1. Purpose and justification
2. Modelling
3. Construction
4. Cloning and Integration
5. Testing and Characterising

1. . Wiki

Labbook 1. Aim 2. Materials and Methods 3. Procedure/Protocol 4. Results 5. Conclusion Everyone needs to carry out this protocol above Photographs add and label them

1. . Presentation