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Team:Newcastle/Meetings/12 July 2010
From 2010.igem.org
(Difference between revisions)
| Line 5: | Line 5: | ||
*'''SR1''' - finished! | *'''SR1''' - finished! | ||
| - | *'''YneA''' - biobrick nearly coming back | + | *'''YneA''' - biobrick nearly coming back - our most important biobrick! |
*'''LacI''' - not sure how to test this biobrick | *'''LacI''' - not sure how to test this biobrick | ||
*'''2 for Swarming''' (sfp and swrA) - cloning strategies nearly finished | *'''2 for Swarming''' (sfp and swrA) - cloning strategies nearly finished | ||
| Line 13: | Line 13: | ||
So only the cloning strategy for SR1 is finished. 7 more to go! | So only the cloning strategy for SR1 is finished. 7 more to go! | ||
| - | + | ===Allocation of team members:=== | |
| - | *YneA | + | *'''YneA''' - Phil, Rach, Deena, Younus |
| - | *Swarming (2) - Harsh, Alan, Steve, Da | + | *'''Swarming (2)''' - Harsh, Alan, Steve, Da |
| - | *LacI - Phil, Younus, Rachel | + | *'''LacI''' - Phil, Younus, Rachel |
| - | *Subtilin (2) - Harsh, Alan, Steve, Deena | + | *'''Subtilin (2)''' - Harsh, Alan, Steve, Deena |
| - | *RocF - Harsh, Alan, Steve, Da | + | *'''RocF''' - Harsh, Alan, Steve, Da |
Revision as of 08:55, 13 July 2010
Chair: Da, Minutes: Younus, Comp
Contents |
Cloning Strategies
There are 8 cloning strategies which are required:
- SR1 - finished!
- YneA - biobrick nearly coming back - our most important biobrick!
- LacI - not sure how to test this biobrick
- 2 for Swarming (sfp and swrA) - cloning strategies nearly finished
- 2 for Subtilin (immunity and production) - cloning strategies nearly finished
- RocF (need to use Gibson method) - not started
So only the cloning strategy for SR1 is finished. 7 more to go!
Allocation of team members:
- YneA - Phil, Rach, Deena, Younus
- Swarming (2) - Harsh, Alan, Steve, Da
- LacI - Phil, Younus, Rachel
- Subtilin (2) - Harsh, Alan, Steve, Deena
- RocF - Harsh, Alan, Steve, Da
5 parts to a Cloning Strategy:
- Purpose and justification
- Modelling
- Construction
- Cloning and Integration
- Testing and Characterising
Wiki
Labbook
- . Aim
- . Materials and Methods
- . Procedure/Protocol
- . Results
- . Conclusion
Everyone needs to carry out this protocol above Photographs add and label them In charge of wiki - Deena and Rachel (everyone everyday they have to send the links to both of them to check)
20th July - UK iGEM Gathering at Newcastle!
Presentation
Introduction - Rach, Deena, Jannetta
- Cover Page
- Picture of standing us up
- Introduction of our iGEM project - using BS to repair concrete (2 mins)
General Outlook (Harsh, Da, Phil) (5 mins):
- Starting with bioreactor
- Transformation into spores
- Transportation
- Spraying on building
- Germination
- sensing crack + non-environmental kill switch
- swarming
- end of crack signalling = QS
- Filamentous cells
- CaCO3
- Glue
- Kill Switch
BioBrick Discussion (Alan, Younus, Steve) (8 mins altogether; 2 mins each):
- Swarming
- Subtillin
- Filamentous cells - YneA
- CaCO3 - Urease
(5 mins remaining)
Whats next... 'Save the babies!!!'
Minideadlines:
- Cloning Strategies
- YneA
- One sequence for sfp primers and coding sequence ready (Goksel)
- Subtilin just immunity
- Finish Wiki updates Sunday 18th
- Team page on Wiki:
- Add to list of advisors: Colin Harwood, Colin Daviy, Wendy Smith and Goksel Misrili
- Deena, Phil and Younus to write about yourself + pictures
Action Points
Da to do everything, thanks
