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Team:Newcastle/Meetings/12 July 2010
From 2010.igem.org
(Difference between revisions)
| Line 40: | Line 40: | ||
#'''Presentation''' | #'''Presentation''' | ||
| - | Cover Page | + | *Cover Page |
| - | Picture of standing us up | + | *Picture of standing us up |
| - | Introduction of our iGEM project - using BS to repair concrete (2 mins) | + | *Introduction of our iGEM project - using BS to repair concrete (2 mins) |
'''General Outlook''' (5 mins): | '''General Outlook''' (5 mins): | ||
| - | Starting with bioreactor | + | *Starting with bioreactor |
| - | Transformation into spores | + | *Transformation into spores |
| - | Transportation | + | *Transportation |
| - | Spraying on building | + | *Spraying on building |
| - | Germination | + | *Germination |
| - | sensing crack + non-environmental kill switch | + | *sensing crack + non-environmental kill switch |
| - | swarming | + | *swarming |
| - | end of crack signalling = QS | + | *end of crack signalling = QS |
| - | Filamentous cells | + | *Filamentous cells |
| - | CaCO3 | + | *CaCO3 |
| - | Glue | + | *Glue |
| - | Kill Switch | + | *Kill Switch |
'''BioBrick Discussion''' (8 mins altogether; 2 mins each): | '''BioBrick Discussion''' (8 mins altogether; 2 mins each): | ||
| - | Swarming | + | *Swarming |
| - | Subtillin | + | *Subtillin |
| - | Filamentous cells - YneA | + | *Filamentous cells - YneA |
| - | CaCo3 - Urease | + | *CaCo3 - Urease |
(5 mins remaining) | (5 mins remaining) | ||
'''Whats next'''... | '''Whats next'''... | ||
Revision as of 15:27, 12 July 2010
Chair: Da, Minutes: Younus, Comp
- . Cloning Strategies
- SR1 - Finished
- RocF not started - need to use Gibson method;
- YneA
- 2 for Swarming (sfp and swrA)
- LacI
- 2 for Subtilin (Immunity and Production)
Only finished one, SR1. 7 more to go!
Next: swarming and subtilin nearly finished, YneA nearly coming back and LacI brick not sure how to test. Why is testing LacI hard?
- YneA - our most important BB - Phil, Rach, Deena, Younus
- Swarming (2) - Harsh, Alan, Steve, Da
- LacI - Phil, Younus, Rachel
- Subtilin (2) - Harsh, Alan, Steve, Deena
- RocF - Harsh, Alan, Steve, Da
5 parts to a Cloning Strategy
- Purpose and justification
- Modelling
- Construction
- Cloning and Integration
- Testing and Characterising
- . Wiki
Labbook
- . Aim
- . Materials and Methods
- . Procedure/Protocol
- . Results
- . Conclusion
Everyone needs to carry out this protocol above Photographs add and label them In charge of wiki - Deena and Rachel (everyone everyday they have to send the links to both of them to check)
- Presentation
- Cover Page
- Picture of standing us up
- Introduction of our iGEM project - using BS to repair concrete (2 mins)
General Outlook (5 mins):
- Starting with bioreactor
- Transformation into spores
- Transportation
- Spraying on building
- Germination
- sensing crack + non-environmental kill switch
- swarming
- end of crack signalling = QS
- Filamentous cells
- CaCO3
- Glue
- Kill Switch
BioBrick Discussion (8 mins altogether; 2 mins each):
- Swarming
- Subtillin
- Filamentous cells - YneA
- CaCo3 - Urease
(5 mins remaining)
Whats next...
