Team:Newcastle/Meetings/12 July 2010

From 2010.igem.org

Revision as of 15:26, 12 July 2010

Chair: Da, Minutes: Younus, Comp

1. . Cloning Strategies

• SR1 - Finished
• RocF not started - need to use Gibson method;
• YneA
• 2 for Swarming (sfp and swrA)
• LacI
• 2 for Subtilin (Immunity and Production)

Only finished one, SR1. 7 more to go!

Next: swarming and subtilin nearly finished, YneA nearly coming back and LacI brick not sure how to test. Why is testing LacI hard?

• YneA - our most important BB - Phil, Rach, Deena, Younus
• Swarming (2) - Harsh, Alan, Steve, Da
• LacI - Phil, Younus, Rachel
• Subtilin (2) - Harsh, Alan, Steve, Deena
• RocF - Harsh, Alan, Steve, Da

5 parts to a Cloning Strategy

1. Purpose and justification
2. Modelling
3. Construction
4. Cloning and Integration
5. Testing and Characterising

1. . Wiki

Labbook

1. . Aim
2. . Materials and Methods
3. . Procedure/Protocol
4. . Results
5. . Conclusion

Everyone needs to carry out this protocol above Photographs add and label them In charge of wiki - Deena and Rachel (everyone everyday they have to send the links to both of them to check)

1. Presentation

Cover Page Picture of standing us up Introduction of our iGEM project - using BS to repair concrete (2 mins)

General Outlook (5 mins): Starting with bioreactor Transformation into spores Transportation Spraying on building Germination sensing crack + non-environmental kill switch swarming end of crack signalling = QS Filamentous cells CaCO3 Glue Kill Switch

BioBrick Discussion (8 mins altogether; 2 mins each): Swarming Subtillin Filamentous cells - YneA CaCo3 - Urease

(5 mins remaining)

Whats next...