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From 2010.igem.org

Revision as of 15:10, 12 July 2010 (view source) Yessa (Talk | contribs) ← Older edit		Revision as of 15:17, 12 July 2010 (view source) Yessa (Talk | contribs) Newer edit →
Line 30:		Line 30:
	#. Wiki		#. Wiki
	Labbook		Labbook
-	1. Aim	+	#. Aim
-	2. Materials and Methods	+	#. Materials and Methods
-	3. Procedure/Protocol	+	#. Procedure/Protocol
-	4. Results	+	#. Results
-	5. Conclusion	+	#. Conclusion
	Everyone needs to carry out this protocol above		Everyone needs to carry out this protocol above
	Photographs add and label them		Photographs add and label them
		+	In charge of wiki - Deena and Rachel (everyone everyday they have to send the links to both of them to check)
	#. Presentation		#. Presentation
		+	Introduction of our iGEM project - using BS to repair concrete
		+	Starting with bioreactor
		+	Transformation into spores
		+	Transportation
		+	Spraying on building
		+	Germination
		+	sensing crack + non-environmental kill switch
		+	swarming
		+	end of crack signalling = QS
		+	Filamentous cells
		+	CaCO3

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Revision as of 15:17, 12 July 2010

Chair: Da, Minutes: Younus, Comp

1. . Cloning Strategies

• SR1 - Finished
• RocF not started - need to use Gibson method;
• YneA
• 2 for Swarming (sfp and swrA)
• LacI
• 2 for Subtilin (Immunity and Production)

Only finished one, SR1. 7 more to go!

Next: swarming and subtilin nearly finished, YneA nearly coming back and LacI brick not sure how to test. Why is testing LacI hard?

• YneA - our most important BB - Phil, Rach, Deena, Younus
• Swarming (2) - Harsh, Alan, Steve, Da
• LacI - Phil, Younus, Rachel
• Subtilin (2) - Harsh, Alan, Steve, Deena
• RocF - Harsh, Alan, Steve, Da

5 parts to a Cloning Strategy

1. Purpose and justification
2. Modelling
3. Construction
4. Cloning and Integration
5. Testing and Characterising

1. . Wiki

Labbook

1. . Aim
2. . Materials and Methods
3. . Procedure/Protocol
4. . Results
5. . Conclusion

Everyone needs to carry out this protocol above Photographs add and label them In charge of wiki - Deena and Rachel (everyone everyday they have to send the links to both of them to check)

1. . Presentation

Introduction of our iGEM project - using BS to repair concrete Starting with bioreactor Transformation into spores Transportation Spraying on building Germination sensing crack + non-environmental kill switch swarming end of crack signalling = QS Filamentous cells CaCO3