Team:Newcastle/IPTG INduction

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Aim

The aim of this protocol is to prepare slides for the Bacillus subtilis 168 cells which have yneA insertion and are induced by IPTG at various concentrations.

Materials

  1. Flasks
  2. Colonies of Bacillus subtilis 168 cells with different plasmid insertions.
  3. Inoculation Loop
  4. Shaking incubator
  5. 1M IPTG stock solution
  6. Appropriate antibiotic solutions
  7. 1.2% agarose
  8. 12 well glass slide
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