Revision as of 10:12, 30 July 2010 by Shethharsh08 (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Growing an Overnight Culture

Materials Required

• LB Broth
• Colonies of bacteria
• Flame loop
• Universal tube
• Pipette

Procedures

1. 5ml of LB broth is pipetted into the universal tube.
2. The flame loop is used to pick a colony present on the agar plate.
3. The colony is then mixed into the LB Broth in the universal tube.
4. The culture is left overnight at 37°C (E.coli, B.subtilis lower temp)

Go back to our Protocol List