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Team:Newcastle/Growing a liquid culture
From 2010.igem.org
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Overnight liquid culture
Materials required
- Universal tube
- Relevant antibiotics
- LB broth
- Relevant colonies
Procedures
- Transfer 5 ml of LB broth to a universal tube
- Pick a single colony and innoculate it
- Grow overnight in 37°C(E.coli, Bacillus subtilis at a lower temperature 20-30°C) with constant shaking
   
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