Team:Newcastle/Glycerol stocks
From 2010.igem.org
(Difference between revisions)
Swoodhouse (Talk | contribs) |
Swoodhouse (Talk | contribs) (→Making glycerol stocks) |
||
Line 6: | Line 6: | ||
* 1.5ml microcentrifuge tubes | * 1.5ml microcentrifuge tubes | ||
- | * | + | * Glycerol |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
==Protocol== | ==Protocol== | ||
# Inoculate 5ml THY Broth with a single colony and incubate, stationary at 37°C for 8 hours. | # Inoculate 5ml THY Broth with a single colony and incubate, stationary at 37°C for 8 hours. | ||
- | # 0. | + | # 0.5ml culture mixed with 0.5ml of sterile 50% glycerol (filter sterlise). |
+ | # Store at -80°C in microcentrifuge tubes. | ||
+ | |||
'''Go back to our [[Team:Newcastle/Protocol list|Protocol List]]''' | '''Go back to our [[Team:Newcastle/Protocol list|Protocol List]]''' | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} |
Latest revision as of 10:28, 19 September 2010
|
Making glycerol stocks
Materials
- 1.5ml microcentrifuge tubes
- Glycerol
Protocol
- Inoculate 5ml THY Broth with a single colony and incubate, stationary at 37°C for 8 hours.
- 0.5ml culture mixed with 0.5ml of sterile 50% glycerol (filter sterlise).
- Store at -80°C in microcentrifuge tubes.
Go back to our Protocol List