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	# 2.5µl backward primer		# 2.5µl backward primer
	# 1µl template DNA		# 1µl template DNA
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		+	====Conditions for ThermoCycler:====

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Revision as of 13:38, 26 July 2010

Colony PCR

Materials to add accordingly:

1. 37.5µl of distilled H2O
2. 10µl of 5x GoTaq Buffer
3. Nucleotide DNTP
4. 2.5µl forward primer
5. 2.5µl backward primer
6. 1µl template DNA

Conditions for ThermoCycler: