Team:Newcastle/Arginine test

From 2010.igem.org

(Difference between revisions)
(Procedures)
(Materials Required)
 
Line 10: Line 10:
* Bursen Burner
* Bursen Burner
* Universal Tube
* Universal Tube
-
* pH measuring stick
+
* pH indicator paper
==Procedures==
==Procedures==

Latest revision as of 20:58, 27 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Arginine Test

Materials Required

  • Plate containing Bacillus subtilis 168 colonies.
  • Flame (streaking) loop
  • LB media consisting of arginine
  • Auto pipette
  • Bursen Burner
  • Universal Tube
  • pH indicator paper

Procedures

  • Perform the experiment using aseptic technique.
  • Transfer B. subtilis 168 colonies into universal tubes containing 5 ml of LB media and allowed to grow overnight at 37° C.
  • Transfer 1 ml of the overnight culture to another universal tube containing 4 ml of the following media:
  1. Control (1) - LB media
  2. Control (2) - LB media with 10 mM of arginine
  3. Control (3) - LB media plus B. subtilis 168
  4. Test (1) - LB media with 10 mM of arginine plus B. subtilis 168
  5. Test (2) - LB media with 10 mM of arginine plus B. subtilis 168
  • Incubate the culture at 37° C with shaking.
  • Record the pH at every 30 min interval.Use 20 ul of the culture and measure the pH using the pH indicator paper.


Go back to our Protocol List

Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon