Team:Newcastle/Arginine test

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===Materials Required===
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{{Team:Newcastle/mainbanner}}
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* Plate consisting of ''Bacillus subtilis'' 168 colonies.
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* Flame (streaking) Loop
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=Arginine Test=
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* LB media consisting arginine and ampicillin
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==Materials Required==
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* Plate containing ''Bacillus subtilis'' 168 colonies.
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* Flame (streaking) loop
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* LB media consisting of  arginine
* Auto pipette
* Auto pipette
* Bursen Burner
* Bursen Burner
* Universal Tube
* Universal Tube
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* pH indicator paper
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===Procedure===
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==Procedures==
* Perform the experiment using aseptic technique.
* Perform the experiment using aseptic technique.
* Transfer ''B. subtilis'' 168 colonies into universal tubes containing 5 ml of LB media and allowed to grow overnight at 37° C.
* Transfer ''B. subtilis'' 168 colonies into universal tubes containing 5 ml of LB media and allowed to grow overnight at 37° C.
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#Test (2) - LB media with 10 mM of arginine plus ''B. subtilis'' 168
#Test (2) - LB media with 10 mM of arginine plus ''B. subtilis'' 168
* Incubate the culture at 37° C with shaking.
* Incubate the culture at 37° C with shaking.
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* Record the pH at every 30 min interval.Use 20 ul of the culture and measure the pH using the pH measuring stick.
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* Record the pH at every 30 min interval.Use 20 ul of the culture and measure the pH using the pH indicator paper.
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'''Go back to our [[Team:Newcastle/Protocol list|Protocol List]]'''
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Latest revision as of 20:58, 27 October 2010

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Arginine Test

Materials Required

  • Plate containing Bacillus subtilis 168 colonies.
  • Flame (streaking) loop
  • LB media consisting of arginine
  • Auto pipette
  • Bursen Burner
  • Universal Tube
  • pH indicator paper

Procedures

  • Perform the experiment using aseptic technique.
  • Transfer B. subtilis 168 colonies into universal tubes containing 5 ml of LB media and allowed to grow overnight at 37° C.
  • Transfer 1 ml of the overnight culture to another universal tube containing 4 ml of the following media:
  1. Control (1) - LB media
  2. Control (2) - LB media with 10 mM of arginine
  3. Control (3) - LB media plus B. subtilis 168
  4. Test (1) - LB media with 10 mM of arginine plus B. subtilis 168
  5. Test (2) - LB media with 10 mM of arginine plus B. subtilis 168
  • Incubate the culture at 37° C with shaking.
  • Record the pH at every 30 min interval.Use 20 ul of the culture and measure the pH using the pH indicator paper.


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