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Team:Newcastle/9 September 2010 - Revision history
2024-03-29T13:36:53Z
Revision history for this page on the wiki
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Yessa: Replacing page with '{{Team:Newcastle/mainbanner}}
{{Team:Newcastle/footer}}'
2010-10-28T02:22:01Z
<p>Replacing page with '{{Team:Newcastle/mainbanner}} {{Team:Newcastle/footer}}'</p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">=Characterization of ''yneA''=</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">==Aim==</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">The aim of this experiment is to prepare slides for the ''Bacillus subtilis'' 168 cells which have ''yneA'' ion the plasmid (pMutin4 and pMap65)under ''lacI'' regulation and are induced by IPTG at various concentrations.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">==Materials and Protocol==</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Please refer to: [[Team:Newcastle/IPTG INduction|Slide preparation for IPTG induced cells]] for materials and protocol.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">For this experiment we used the following cell colonies:</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">#''Bacillus subtilis'' 168 </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">#''Bacillus subtilis'' 168 with pMutin4 having ''yneA'' insert</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">#''Bacillus subtilis'' 168 tith pMap65 having ''yneA'' insert</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Grow them overnight.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">==Result and Conclusion==</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Please refer to: [[Team:Newcastle/10_September_2010|10.10.2010]] for result and conclusion of the experiment.</del></div></td><td colspan="2"> </td></tr>
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Yessa
http://2010.igem.org/wiki/index.php?title=Team:Newcastle/9_September_2010&diff=153487&oldid=prev
Shethharsh08: /* Aim */
2010-10-26T03:09:22Z
<p><span class="autocomment">Aim</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Aim==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Aim==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The aim of this experiment is to prepare slides for the ''Bacillus subtilis'' 168 cells which have ''yneA'' <del class="diffchange diffchange-inline">integrated into the chromosome or is on </del>the plasmid (pMutin4 and pMap65) and are induced by IPTG at various concentrations.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The aim of this experiment is to prepare slides for the ''Bacillus subtilis'' 168 cells which have ''yneA'' <ins class="diffchange diffchange-inline">ion </ins>the plasmid (pMutin4 and pMap65)<ins class="diffchange diffchange-inline">under ''lacI'' regulation </ins>and are induced by IPTG at various concentrations.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Materials and Protocol==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Materials and Protocol==</div></td></tr>
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Shethharsh08
http://2010.igem.org/wiki/index.php?title=Team:Newcastle/9_September_2010&diff=153371&oldid=prev
Shethharsh08: /* Result and Conclusion */
2010-10-26T02:58:22Z
<p><span class="autocomment">Result and Conclusion</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Result and Conclusion==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Result and Conclusion==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Please refer to: [[Team:Newcastle/<del class="diffchange diffchange-inline">11_September_2010</del>|<del class="diffchange diffchange-inline">11</del>.10.2010]] for result and conclusion of the experiment.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Please refer to: [[Team:Newcastle/<ins class="diffchange diffchange-inline">10_September_2010</ins>|<ins class="diffchange diffchange-inline">10</ins>.10.2010]] for result and conclusion of the experiment.</div></td></tr>
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Shethharsh08
http://2010.igem.org/wiki/index.php?title=Team:Newcastle/9_September_2010&diff=153367&oldid=prev
Shethharsh08: /* Result and Conclusion */
2010-10-26T02:57:14Z
<p><span class="autocomment">Result and Conclusion</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Result and Conclusion==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Result and Conclusion==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Please refer to: [[]] for result and conclusion of the experiment.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Please refer to: [[<ins class="diffchange diffchange-inline">Team:Newcastle/11_September_2010|11.10.2010</ins>]] for result and conclusion of the experiment.</div></td></tr>
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Shethharsh08
http://2010.igem.org/wiki/index.php?title=Team:Newcastle/9_September_2010&diff=153357&oldid=prev
Shethharsh08: /* Result and Conclusion */
2010-10-26T02:55:54Z
<p><span class="autocomment">Result and Conclusion</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Result and Conclusion==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Result and Conclusion==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Please refer to: [[<del class="diffchange diffchange-inline">https://2010.igem.org/Team:Newcastle/11_September_2010|11.10.2010</del>]] for result and conclusion of the experiment.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Please refer to: [[]] for result and conclusion of the experiment.</div></td></tr>
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Shethharsh08
http://2010.igem.org/wiki/index.php?title=Team:Newcastle/9_September_2010&diff=153348&oldid=prev
Shethharsh08 at 02:55, 26 October 2010
2010-10-26T02:55:26Z
<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Newcastle/mainbanner}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Newcastle/mainbanner}}</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">=Characterization of ''yneA''=</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Aim==</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The aim of this experiment is to prepare slides for the ''Bacillus subtilis'' 168 cells which have ''yneA'' integrated into the chromosome or is on the plasmid (pMutin4 and pMap65) and are induced by IPTG at various concentrations.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Materials and Protocol==</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Please refer to: [[Team:Newcastle/IPTG INduction|Slide preparation for IPTG induced cells]] for materials and protocol.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">For this experiment we used the following cell colonies:</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">#''Bacillus subtilis'' 168 </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">#''Bacillus subtilis'' 168 with pMutin4 having ''yneA'' insert</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">#''Bacillus subtilis'' 168 tith pMap65 having ''yneA'' insert</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Grow them overnight.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Result and Conclusion==</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Please refer to: [[https://2010.igem.org/Team:Newcastle/11_September_2010|11.10.2010]] for result and conclusion of the experiment.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Newcastle/footer}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Newcastle/footer}}</div></td></tr>
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Shethharsh08
http://2010.igem.org/wiki/index.php?title=Team:Newcastle/9_September_2010&diff=152828&oldid=prev
RachelBoyd: New page: {{Team:Newcastle/mainbanner}} {{Team:Newcastle/footer}}
2010-10-26T02:04:22Z
<p>New page: {{Team:Newcastle/mainbanner}} {{Team:Newcastle/footer}}</p>
<p><b>New page</b></p><div>{{Team:Newcastle/mainbanner}}<br />
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RachelBoyd