Team:Newcastle/9 September 2010

From 2010.igem.org

(Difference between revisions)
(Result and Conclusion)
(Aim)
Line 5: Line 5:
==Aim==
==Aim==
-
The aim of this experiment is to prepare slides for the ''Bacillus subtilis'' 168 cells which have ''yneA'' integrated into the chromosome or is on the plasmid (pMutin4 and pMap65) and are induced by IPTG at various concentrations.
+
The aim of this experiment is to prepare slides for the ''Bacillus subtilis'' 168 cells which have ''yneA'' ion the plasmid (pMutin4 and pMap65)under ''lacI'' regulation and are induced by IPTG at various concentrations.
==Materials and Protocol==
==Materials and Protocol==

Revision as of 03:09, 26 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Contents

Characterization of yneA

Aim

The aim of this experiment is to prepare slides for the Bacillus subtilis 168 cells which have yneA ion the plasmid (pMutin4 and pMap65)under lacI regulation and are induced by IPTG at various concentrations.

Materials and Protocol

Please refer to: Slide preparation for IPTG induced cells for materials and protocol. For this experiment we used the following cell colonies:

  1. Bacillus subtilis 168
  2. Bacillus subtilis 168 with pMutin4 having yneA insert
  3. Bacillus subtilis 168 tith pMap65 having yneA insert

Grow them overnight.

Result and Conclusion

Please refer to: 10.10.2010 for result and conclusion of the experiment.



Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon